Coupling osmium complexes to epoxy-functionalised polymers to provide mediated enzyme electrodes for glucose oxidation.
Coupling osmium complexes to epoxy-functionalised polymers to provide mediated enzyme electrodes for glucose oxidation.
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DOI：
10.1016/j.bios.2012.11.036
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发表时间：
2013-05
影响因子：
12.6
通讯作者：
Peter Ó Conghaile;Sascha Pöller;Domhnall MacAodha;W. Schuhmann;D. Leech
中科院分区：
文献类型：
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作者：
Peter Ó Conghaile;Sascha Pöller;Domhnall MacAodha;W. Schuhmann;D. Leech
新合成的锇络合物改性的氧化还原聚合物进行了测试，作为葡萄糖氧化酶电极的应用，生物传感器或生物燃料电池的调解员的潜在应用。将含有胺官能团的锇络合物偶联到可变组成的环氧官能化聚合物上提供了一系列氧化还原聚合物，这些氧化还原聚合物在氧化还原电位和物理化学性质上可能存在变化。作为葡萄糖氧化的介体的氧化还原聚合物的性质进行了研究，通过共固定到石墨与葡萄糖氧化酶或FAD依赖性葡萄糖脱氢酶使用的交联剂的范围内，并在存在和不存在的多壁碳纳米管。通过固定[P20-Os（2，2 ′-bipyridine）2（4-aminomethyl pyridine）Cl]. PF 6、碳纳米管和葡萄糖氧化酶制备的电极在0.45V vs. Ag/AgCl下对含有100 mM葡萄糖的PBS显示出高达560μAcm− 2的葡萄糖氧化电流密度。通过交联[P20-Os（4，4 ′-dimethoxy-2，2 ′-bipyridine）2（4-aminomethyl pyridine）Cl]. PF 6（一种FAD依赖性葡萄糖脱氢酶）和碳纳米管制备的薄膜在5 mM葡萄糖中在0.2V vs. Ag/AgCl下实现了215μAcm− 2的电流密度，显示出应用于葡萄糖氧化生物传感器或生物燃料电池的前景。
Newly synthesised osmium complex-modified redox polymers were tested for potential application as mediators in glucose oxidising enzyme electrodes for application to biosensors or biofuel cells. Coupling of osmium complexes containing amine functional groups to epoxy-functionalised polymers of variable composition provides a range of redox polymers with variation possible in redox potential and physicochemical properties. Properties of the redox polymers as mediators for glucose oxidation were investigated by co-immobilisation onto graphite with glucose oxidase or FAD-dependent glucose dehydrogenase using a range of crosslinkers and in the presence and absence of multiwalled carbon nanotubes. Electrodes prepared by immobilising [P20-Os(2,2′-bipyridine)2(4-aminomethylpyridine)Cl].PF6, carbon nanotubes and glucose oxidase exhibit glucose oxidation current densities as high as 560μAcm−2for PBS containing 100mM glucose at 0.45V vs. Ag/AgCl. Films prepared by crosslinking [P20-Os(4,4′-dimethoxy-2,2′-bipyridine)2(4-aminomethylpyridine)Cl].PF6, an FAD-dependent glucose dehydrogenase, and carbon nanotubes achieve current densities of 215μAcm−2in 5mM glucose at 0.2V vs. Ag/AgCl, showing some promise for application to glucose oxidising biosensors or biofuel cells.