The evaluation of feeding, mortality and oviposition of poultry red mite ( Dermanyssus gallinae) on aging hens using a high welfare on-hen feeding device.
The evaluation of feeding, mortality and oviposition of poultry red mite ( Dermanyssus gallinae) on aging hens using a high welfare on-hen feeding device.
复制标题
DOI：
10.12688/f1000research.26398.1
复制
发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Nisbet AJ
中科院分区：
文献类型：
--
作者：
Nunn F;Bartley K;Palarea-Albaladejo J;Nisbet AJ
进行了一项研究，以检查当使用高福利、母鸡上螨饲喂装置饲喂时，母鸡年龄对不同生命阶段的鸡皮刺螨[家禽红螨（PRM）]的饲喂能力和死亡率的任何影响。每两周对一组五只罗曼布朗母鸡进行螨摄食测定，所述罗曼布朗母鸡具有含有成虫和次若虫PRM或成虫和原若虫PRM的装置。饲养率和死亡率的每个PRM的生活阶段和产卵的成年女性PRM超过18周的时间进行了评估。有一个显着降低产卵率的女性PRM，因为他们对母鸡的年龄增加。然而，没有明确的趋势之间检测到的所有三个吸血的生命阶段和母鸡年龄的喂养率。同样的结论是关于螨死亡率喂养后的次若虫和成年女性PRM，虽然弱正相关母鸡年龄和前若虫PRM死亡率之间是明显的。这项研究表明，母鸡喂养装置可用于短期研究，以评估新的抗PRM产品（新杀螨剂，疫苗等）以及更长的纵向研究，以确定这种新型抗PRM产品的效果的持久性。它还表明，螨对老年母鸡的吸血比对年轻母鸡的吸血更不能维持PRM种群。与传统的PRM攻击侵染模型相比，这种母鸡上螨饲养装置通过大大减少每个实验组所需的禽类数量以及通过消除禽类长时间暴露于大量螨的需要（这可能导致福利问题）来直接影响减少和改进。本文介绍了这些研究的方法，以及如何组装袋和处理螨之前和之后的喂养试验。
A study was performed to examine any effect of hen age on the feeding ability and mortality of different life-stages of Dermanyssus gallinae [Poultry Red Mite (PRM)] when fed using a high welfare, on-hen mite feeding device. Mite feeding assays were carried out every two weeks on a cohort of five Lohman Brown hens with devices containing adult and deutonymph PRM or adult and protonymph PRM. Feeding rates and mortality of each PRM life stage and oviposition of adult female PRM were evaluated over an 18-week period. There was a significant reduction in oviposition rates of female PRM as they fed on hens of increasing age. However, no clear trend was detected between the feeding rates of all three haematophagous life stages and hen age. The same conclusion was reached regarding mite mortality post-feeding in both deutonymph and adult female PRMs, although a weak positive association was apparent between hen age and protonymph PRM mortality. This study shows that the on-hen feeding device can be used both for short term studies to assess novel anti-PRM products (new acaricides, vaccines etc.) and longer, longitudinal studies to determine longevity of the effects of such novel anti-PRM products. It also demonstrates that blood feeding by mites on older hens is less able to sustain PRM populations than feeding on younger hens. This on-hen mite feeding device directly impacts upon reduction and refinement by greatly reducing the numbers of birds required per experimental group compared to traditional PRM challenge infestation models and by eliminating the need for birds to be exposed to large numbers of mites for extended periods of time that can cause welfare concerns. This paper describes the methodology for these studies and how to assemble pouches and handle mites both before and after feeding assays.