EXPRESSION OF AN EXTRACELLULAR DELETION OF XOTCH DIVERTS CELL FATE IN XENOPUS-EMBRYOS
EXPRESSION OF AN EXTRACELLULAR DELETION OF XOTCH DIVERTS CELL FATE IN XENOPUS-EMBRYOS
复制标题
DOI：
10.1016/0092-8674(93)90247-n
复制
发表时间：
1993-05-21
期刊：
影响因子：
64.5
通讯作者：
KINTNER, CR
中科院分区：
文献类型：
--
作者：
COFFMAN, CR;SKOGLUND, P;KINTNER, CR
Xotch是非洲爪哇的Notch同源物，Notch是一种参与果蝇细胞命运决定的受体。使用细胞外缺失结构XotchDELTAE，我们证明了Xotch在非洲爪哇胚胎中也有类似的作用。广泛的表达会导致背部结构的丧失，以及大脑的扩张和混乱。即使在没有细胞分裂的情况下，单次注射XotchDELTAE也会诱导自主神经和中胚层肥大。XotchDELTAE抑制了表皮和神经脊标记的早期表达，但增强和延长了动物帽子对中胚层和神经诱导的反应。我们的数据提示了Notch同系物的一种功能机制，在这种机制中，它们推迟了分化，并使未确定的细胞有能力对后来的诱导信号做出反应。
Xotch is a Xenopus homolog of Notch, a receptor involved in cell fate decisions in Drosophila. Using an extracellular deletion construct, XotchDELTAE, we show that Xotch has a similar role in Xenopus embryos. Broad expression causes the loss of dorsal structures and the expansion and disorganization of the brain. Single blastomere injections of XotchDELTAE induce autonomous neural and mesodermal hypertrophy, even in the absence of cell division. XotchDELTAE inhibits the early expression of epidermal and neural crest markers yet enhances and extends the response of animal caps to mesodermal and neural induction. Our data suggest a mechanism for the function of Notch homologs in which they delay differentiation and leave undetermined cells competent to respond to later inductive signals.