EXPRESSION AND ACTIVITY OF THE POU TRANSCRIPTION FACTOR SCIP
EXPRESSION AND ACTIVITY OF THE POU TRANSCRIPTION FACTOR SCIP
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DOI：
10.1126/science.1975954
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发表时间：
1990-09-14
期刊：
影响因子：
56.9
通讯作者：
LEMKE, G
中科院分区：
文献类型：
--
作者：
MONUKI, ES;KUHN, R;LEMKE, G
POU蛋白已被证明可以转录激活细胞特异性基因，并参与决定细胞命运。因此，人们认为这些蛋白质通过稳定激活定义特定发育途径的基因在发育中起作用。这里提供了另一种行动方式的证据。推导了周围神经系统发育中的雪旺细胞表达的POU蛋白SCIP的一级结构，并对SCIP的活性进行了研究。在正常发育和神经横断反应中，SCIP表达仅在雪旺细胞分化的前髓鞘期和成髓鞘期的快速细胞分裂期间短暂激活。在共转染实验中，SCIP作为髓磷脂特异性基因的转录抑制因子。
POU proteins have been shown to transcriptionally activate cell-specific genes and to participate in the determination of cell fate. It is therefore thought that these proteins function in development through the stable activation of genes that define specific developmental pathways. Evidence is provided here for an alternative modes of action. The primary structure of SCIP, a POU protein expressed by developing Schwann cells of the peripheral nervous system, was deduced and SCIP activity was studied. Both in normal development and in response to nerve transection SCIP expression was transiently activated only during the period of rapid cell division that separates the premyelinating and myelinating phases of Schwann cell differentiation. In cotransfection assays, SCIP acted as a transcriptional repressor of myelin-specific genes.