In vitro degradation, hemolysis and MC3T3-E1 cell adhesion of biodegradable Mg-Zn alloy
In vitro degradation, hemolysis and MC3T3-E1 cell adhesion of biodegradable Mg-Zn alloy
复制标题
可生物降解镁锌合金的体外降解、溶血和MC3T3-E1细胞粘附
DOI：
10.1016/j.msec.2009.03.001
复制
发表时间：
2009-08-01
影响因子：
7.9
通讯作者：
Bian, Yujun
中科院分区：
文献类型：
--
作者：
Zhang, Shaoxiang;Li, Jianan;Bian, Yujun
本文研究了一种具有专利的mg - 6wt %Zn二元镁合金作为生物医用材料的可降解材料。体外降解实验包括电化学测量和模拟体液（SBF）浸泡试验表明，锌可以提高镁的腐蚀电位和法拉第电荷转移电阻，从而提高其耐腐蚀性。XRD和EDS分析表明，Mg- zn表面的腐蚀产物中含有羟基磷灰石（HA）、Mg (OH)(2)等Mg/Ca磷酸盐，降低了Mg- zn的降解速率。本文还讨论了镁合金的降解过程和腐蚀层的形成机理，即镁的降解副产物Mg2+和OH-与SBF中的磷酸盐和Ca2+反应，使含HA、Mg(OH)(2)等镁取代磷灰石的腐蚀层沉淀在腐蚀坑中并覆盖在镁合金表面。溶血试验发现Mg-Zn溶血率为3.4%，低于ISO 10993-4规定的安全值5%。在细胞培养实验中，培养2 h后，成骨前细胞MC3T3-E1能够在Mg-Zn合金的腐蚀层上粘附扩散，说明尽管DMEM培养液pH值波动，Mg-Zn合金仍能支持成骨前细胞早期在表面的粘附。镁锌合金体外溶血和细胞粘附表现出良好的生物相容性。(C) 2009 Elsevier B.V.版权所有
In this study a kind of patent binary Mg-6 wt.%Zn magnesium alloy was investigated as degradable biomedical material. The results of in vitro degradation including electrochemical measurements and immersion tests in simulated body fluid (SBF) revealed that zinc could elevate both the corrosion potential and Faraday charge transfer resistance of magnesium and thus improve the corrosion resistance. XRD and EDS analysis proved that the corrosion products on the surface of Mg-Zn contained hydroxyapatite (HA), Mg (OH)(2) and other Mg/Ca phosphates, which could reduce the degradation rate. The degradation process of magnesium alloy and the mechanism of corrosion layer formation were also discussed in this work, i.e. the byproducts of degradation of magnesium, Mg2+ and OH-, reacted with the phosphate and Ca2+ in the SBF, thus the corrosion layer containing HA, Mg(OH)(2) and other magnesium-substituted apatite precipitated in corrosion pits and covered the surface of magnesium alloy.The hemolysis test found that the hemolysis rate of Mg-Zn was 3.4%, which is lower than the safe value of 5% according to ISO 10993-4. For the cell culture experiments, after 2 h incubation the pre-osteoblastic cell MC3T3-E1 was able to adhere and spread on the corrosion layer of Mg-Zn alloy, indicating that despite the fluctuation of pH value of DMEM culture solution, Mg-Zn alloy could still support the earlier adhesion of pre-osteoblastic cells on the surface. Hemolysis and adhesion of cells display good biocompatibility of Mg-Zn alloy in vitro. (C) 2009 Elsevier B.V. All rights reserved.