Purification of sinus gland peptides having vitellogenesis-inhibiting activity from the whiteleg shrimp Litopenaeus vannamei
Purification of sinus gland peptides having vitellogenesis-inhibiting activity from the whiteleg shrimp Litopenaeus vannamei
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DOI：
10.1007/s10126-006-6151-0
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发表时间：
2007-05-01
影响因子：
3
通讯作者：
Wilder, Marcy N.
中科院分区：
文献类型：
--
作者：
Tsutsui, Naoaki;Ohira, Tsuyoshi;Wilder, Marcy N.
甲壳动物中的卵黄发生抑制激素（VIH）属于甲壳动物高血糖激素（CHH）家族。为了鉴定凡纳滨对虾中的多种VIH分子，纯化了7种CHH家族肽，命名为Liv-SGP-A、-B、-C、-D、-E、-F和-G。通过反相HPLC和N-末端氨基酸测序鉴定。这些肽对卵黄蛋白原mRNA水平的剂量反应效应进行了研究，使用在体外孵育的卵巢片段的库鲁马对虾囊对虾。Liv-SGP-D对卵黄蛋白原mRNA表达无明显抑制作用，其余6种肽对卵黄蛋白原mRNA表达有明显的抑制作用，但抑制作用大小顺序为Liv-SGP-C，-F，-G > -A，-B > -E。Liv-SGP-G是窦腺中含量最丰富的CHH家族多肽，具有较强的卵黄发生抑制活性。作为详细结构分析的结果，确定了其完整的一级结构;它由72个氨基酸残基组成。具有酰胺化的C末端。
Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. To characterize multiple VIH molecules in the whiteleg shrimp Litopenaeus vannamei, seven CHH-family peptides designated as Liv-SGP-A, -B, -C, -D, -E, -F, and -G were purified. by reversed-phase HPLC and identified by N-terminal amino acid sequencing. The dose-response effects of these peptides on vitellogenin mRNA levels were examined using in vitro incubation of ovarian fragments of the kuruma prawn Marsupenaeus japonicus. Liv-SGP-D showed no significant inhibitory activities, while the other six peptides significantly reduced vitellogenin mRNA levels, however, with differing efficacies, in the order of Liv-SGP-C, -F, -G > -A, -B > -E. Liv-SGP-G was the most abundant CHH-family peptide in the sinus gland and showed strong vitellogenesis-inhibiting activity. As a result of detailed structural analysis, its complete primary structure was determined; it consisted of 72 amino acid residues and. possesses an amidated C-terminus.