Photodynamic therapy by in situ nonlinear photon conversion
Photodynamic therapy by in situ nonlinear photon conversion
复制标题
通过原位非线性光子转换进行光动力治疗
DOI：
10.1038/nphoton.2014.90
复制
发表时间：
2014-06-01
期刊：
影响因子：
35
通讯作者：
Prasad, P. N.
中科院分区：
文献类型：
--
作者：
Kachynski, A. V.;Pliss, A.;Prasad, P. N.
在光动力疗法中，光被治疗剂（光敏剂）吸收以产生活性氧，然后活性氧局部杀死患病细胞。在这里，我们报告了一种新形式的光动力疗法，其中近红外激光辐射与生物介质的非线性光学相互作用原位产生光，该光福尔斯落入光敏剂的吸收带内。使用近红外辐射，然后上转换为可见光或紫外光，提供了深入的组织穿透，从而克服了治疗中的主要障碍。通过建模和实验，我们证明了一种已知的光敏剂，二氢卟酚e6，在原位非线性光学上转换的近红外激光辐射使用二次谐波产生的胶原蛋白和四波混频，包括相干反斯托克斯拉曼散射，产生的细胞生物分子的激活。相干反斯托克斯拉曼散射/四波混频在体外光动力治疗的引入增加了效率的两倍相比，单独的双光子光动力治疗，而二次谐波产生提供了五倍的增加。
In photodynamic therapy, light is absorbed by a therapy agent (photosensitizer) to generate reactive oxygen, which then locally kills diseased cells. Here, we report a new form of photodynamic therapy in which nonlinear optical interactions of near-infrared laser radiation with a biological medium in situ produce light that falls within the absorption band of the photosensitizer. The use of near-infrared radiation, followed by upconversion to visible or ultraviolet light, provides deep tissue penetration, thus overcoming a major hurdle in treatment. By modelling and experiment, we demonstrate activation of a known photosensitizer, chlorin e6, by in situ nonlinear optical upconversion of near-infrared laser radiation using second-harmonic generation in collagen and four-wave mixing, including coherent anti-Stokes Raman scattering, produced by cellular biomolecules. The introduction of coherent anti-Stokes Raman scattering/four-wave mixing to photodynamic therapy in vitro increases the efficiency by a factor of two compared to two-photon photodynamic therapy alone, while second-harmonic generation provides a fivefold increase.