Regulation of gonadotropin receptors and gonadotropin responses in a clonal strain of Leydig tumor cells by epidermal growth factor.
Regulation of gonadotropin receptors and gonadotropin responses in a clonal strain of Leydig tumor cells by epidermal growth factor.
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DOI：
10.1016/s0021-9258(19)70116-1
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发表时间：
1981-01
期刊：
影响因子：
--
通讯作者：
M. Ascoli
中科院分区：
文献类型：
--
作者：
M. Ascoli
MA-10系是一种具有人绒毛膜促性腺激素(HCG)和小鼠表皮生长因子(MEGF)受体的间质肿瘤细胞克隆株。这些细胞对人绒毛膜促性腺激素、霍乱毒素和8-溴-腺苷3‘：5’-单磷酸有反应，类固醇产量增加。据报道，MA-10细胞暴露于mEGF后，每个细胞的hCG受体数量显著减少(80%~90%)。HCG受体的丧失伴随着hCG刺激类固醇生成能力的相应降低。但对霍乱毒素和8-溴-腺苷3‘：5’-一磷酸的类固醇生成反应不受影响。其他结果表明，mEGF不是这些细胞的有丝分裂原。
The MA-10 line is a clonal strain of Leydig tumor cells that has receptors for human choriogonadotropin (hCG) and mouse epidermal growth factor (mEGF). These cells respond to hCG, cholera toxin, and 8-Br-adenosine 3':5'-monophosphate with increased steroid production. It is reported herein that exposure of the MA-10 cells to mEGF results in a substantial (80 to 90%) reduction in the number of hCG receptors per cell. The loss of hCG receptors is accompanied by a corresponding reduction in the ability of hCG to stimulate steroidogenesis. The steroidogenic responses to cholera toxin and 8-Br-adenosine 3':5'-monophosphate, however, are not affected. Other results presented show that mEGF is not a mitogen for these cells.