Intratracheal Poly(I:C) Exposure Accelerates the Immunological Disorder of Salivary Glands in Sjogren's-Like NOD/ShiLtJ Mice.
Intratracheal Poly(I:C) Exposure Accelerates the Immunological Disorder of Salivary Glands in Sjogren's-Like NOD/ShiLtJ Mice.
复制标题
气管内暴露 Poly(I:C) 会加速干燥样 NOD/ShiLtJ 小鼠唾液腺的免疫紊乱
DOI：
10.3389/fmed.2021.645816
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发表时间：
2021
影响因子：
3.9
通讯作者：
Dong L
中科院分区：
文献类型：
--
作者：
Hu P;Ming B;Wu X;Cai S;Tang J;Dong Y;Zhou T;Tan Z;Zhong J;Zheng F;Dong L
有证据表明，干燥综合征（SS）与病毒感染有关。本研究的目的是研究呼吸道病毒poly（I：C）参与SS的发病机制和潜在的机制，使用SS样NOD/ShiLtJ（NOD）小鼠模型。5-每隔一天向雌性NOD小鼠体内施用聚（I：C）5次以模拟病毒感染。每8天测定一次毛果芸香碱诱导的唾液分泌。取下颌下腺和肺组织进行病理学检查。我们发现，结肠内给药poly（I：C）显着先进和增强NOD小鼠唾液流速的降低。此外，poly（I：C）处理加重SMG的组织病理学病变和炎性细胞浸润。随着IFN细胞因子和IL-33表达的增加，poly（I：C）处理的NOD小鼠SMG中Th 1活化增强，但Th 17细胞活化在各组中无变化。此外，腹腔注射poly（I：C）可促进肺组织IL-33的表达和T细胞比例的增加，这与SMG的变化一致。因此，腹腔注射poly（I：C）可加重NOD小鼠SMG的免疫功能紊乱。
Evidences have suggested that Sjogren's syndrome (SS) is associated with viral infection. The aim of this study was to investigate the involvement of respiratory viral poly(I:C) in the pathogenesis of SS and potential mechanisms using a SS-like NOD/ShiLtJ (NOD) mouse model. 5-week female NOD mice were intratracheally administered poly(I:C) every other day for 5 times to mimic viral infection. Pilocarpine induced saliva secretion was determined every 8 days. Submandibular glands (SMG) and lungs were harvested for the detection of pathological changes. We found that intratracheal administration of poly(I:C) significantly advanced and enhanced the reduction of saliva flow rate in NOD mice. Furthermore, poly(I:C) treatment aggravated the histopathological lesions and inflammatory cells infiltration in SMG. Accompanied by elevated expression of IFN cytokines and IL-33, Th1 activation was enhanced in SMG of poly(I:C)-treated NOD mice, but Th17 cells activation was unchanged among the groups. In addition, intratracheal poly(I:C) exposure promoted the expression of IL-33 and increased T cells proportion in the lung, which were consistent with the change in SMG. Therefore, intratracheal poly(I:C) exposure aggravated the immunological and function disorder of SMG in NOD mice.