Rejoining of DNA strand breaks is an early nuclear event during the stimulation of quiescent lymphocytes.
Rejoining of DNA strand breaks is an early nuclear event during the stimulation of quiescent lymphocytes.
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DNA 链断裂的重新连接是刺激静止淋巴细胞期间的早期核事件。
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
A. Johnstone
中科院分区：
文献类型：
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作者：
A. Johnstone
由有丝分裂原植物血凝素（PHA）激活静止的人外周血淋巴细胞或纯化的T细胞涉及存在于静止细胞中的DNA断裂的快速重新连接，如通过类核沉降分析和碱中的链解旋速率检测的。ADP-核糖基转移酶（ADPRT）抑制剂以剂量依赖性方式阻止PHA或伴刀豆球蛋白A激活外周淋巴细胞或T细胞，但仅在早期阶段存在。如果以后加入，它们不会影响随后的增殖，也不会抑制淋巴母细胞系的生长。抑制剂减缓DNA断裂的重新连接，但不影响有丝分裂原与细胞表面的结合或PHA刺激的质膜肌醇磷脂的早期周转。由ADPRT调节的DNA断裂和重新连接可能参与控制分化过程中的基因表达。
Activation of quiescent human peripheral blood lymphocytes or purified T cells by the mitogen, phytohemagglutinin (PHA), involves a rapid rejoining of DNA breaks present in the resting cells as detected by both nucleoid sedimentation analysis and rate of strand unwinding in alkali. Inhibitors of the enzyme ADP-ribosyltransferase (ADPRT) prevent activation of peripheral lymphocytes or T cells by PHA or concanavalin A in a dose-dependent manner, but only if present during the early stages. They do not affect subsequent proliferation if added later, nor do they inhibit the growth of lymphoblastoid cell lines. The inhibitors slow the rejoining of DNA breaks but do not affect the binding of mitogen to the cell surface or the early PHA-stimulated turnover of plasma membrane inositol phospholipids. DNA breaking and rejoining, regulated by ADPRT, may be involved in controlling gene expression during differentiation.