Co-expression of reporter genes in the widespread pathogen Eimeria tenella using a double-cassette expression vector strategy
Co-expression of reporter genes in the widespread pathogen Eimeria tenella using a double-cassette expression vector strategy
复制标题
使用双盒表达载体策略在广泛传播的病原体柔嫩艾美耳球虫中共表达报告基因。
DOI：
10.1016/j.ijpara.2011.04.001
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发表时间：
2011-07-01
影响因子：
4
通讯作者：
Suo, Xun
中科院分区：
文献类型：
--
作者：
Yin, Guangwen;Liu, Xianyong;Suo, Xun
The double-cassette expression vector strategy is valuable for many studies, including comparative analysis of the function of promoters and expression of genes in different compartments. In this study, we report co-expression of enhanced yellow fluorescent protein (EYFP) and red fluorescent protein (RFP) in Eimeria tenella transfected with two double-cassette expression vectors, pMIC-EYFP/ACT-RFP and pMIC-EYFP/ACTss-RFP. The results showed that under regulation of the mid promoter, EYFP was expressed in sporulated oocysts but not in unsporulated ones, while under regulation of the actin promoter RFP was expressed in both forms. We found that the signal peptide of Toxoplasma gondii dense granule protein 8 (GRA8) located the RFP expression to the parasitophorous vacuoles of the parasites, the margins of the unsporulated oocysts and the cavities of the sporocysts. The feasibility of co-expression of exogenous proteins in E. tenella is important for the development of transgenic E. tenella as a novel vaccine vector. (C) 2011 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
