Increase in the ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 in saliva from patients with primary Sjogren's syndrome
Increase in the ratio of matrix metalloproteinase-9 to tissue inhibitor of metalloproteinase-1 in saliva from patients with primary Sjogren's syndrome
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DOI：
10.1016/j.cccn.2004.03.006
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发表时间：
2004-07-01
影响因子：
5
通讯作者：
Igarashi, A
中科院分区：
文献类型：
--
作者：
Asatsuma, M;Ito, S;Igarashi, A
背景资料：为探讨原发性干燥综合征（PSS）患者和健康人唾液中基质金属蛋白酶（MMP）与金属蛋白酶组织抑制剂（TIMP）的比值，测定了PSS患者和健康人唾液中MMP-2、9和TIMP-1、2的含量。方法：收集32例患者和26例健康人的刺激性全混合唾液。采用酶联免疫吸附试验（ELISA）和双抗体夹心酶免疫分析法（EIA）检测MMP-2、9和TIMP-1、2水平。酶谱法和反向酶谱法用于鉴定MMPs和TIMPs。结果：患者唾液中MMP-9（明胶酶-B）水平显著升高。MMP-9（ng/ml）：患者231.02 ± 151.77（平均值± S.D.），健康受试者145.87 +/- 111.65（p0.05）。相应地，MMP-9/TIMP-1在患者中（2.60 ± 1.18）比在健康受试者中（1.28 ± 1.11）显著增加（p < 0.01）。结论：本研究发现，pSS患者唾液中MMP-9/TIMP-1和MMP-9水平显著高于健康人。我们的研究结果表明，MMP-9/TIMP-1的增加，而不是MMP-9的增加，在pSS患者的唾液中强烈参与腺和唾液导管组织的破坏。(C)2004 Elsevier B. V.保留所有权利。
Background: To investigate the ratio of matrix metalloproteinase (MMP) to tissue inhibitor of metalloproteinase (TIMP) in primary, Sjogren's syndrome (PSS), patients and healthy subjects MMP-2, 9 and TIMP-1, 2 levels were measured in saliva. Methods: Stimulated whole-mixed saliva was collected from 32 patients and 26 healthy subjects. MMP-2, 9 and TIMP-1, 2 levels were measured using enzyme-linked immunosorbent assay (ELISA) and the sandwich enzyme immunoassay (sandwich EIA). Zymography and reverse zymography were used to identify MMPs and TIMPs. Results: MMP-9 (gelatinise-B) level in saliva was significantly increased in the patients. MMP-9 (ng/ml): patients 231.02 +/- 151.77 (mean +/- S.D.), healthy subjects 145.87 +/- 111.65 (p0.05). Accordingly, MMP-9/TIMP-1 was greatly increased in the patients (2.60 +/- 1.18) than in the healthy subjects (1.28 +/- 1.11) (p < 0.01). Conclusion: This study found that MMP-9/TIMP-1 and MMP-9 levels in the saliva were significantly higher in pSS patients than those in healthy subjects. Our results indicate that the increase in MMP-9/TIMP-1, rather than the increase in MMP-9, in pSS patients' saliva is strongly involved in destruction of glandular and salivary duct tissues. (C) 2004 Elsevier B.V. All rights reserved.