Effects of chronic mental stress and atherogenic diet on the immune inflammatory environment in mouse aorta.
Effects of chronic mental stress and atherogenic diet on the immune inflammatory environment in mouse aorta.
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慢性精神应激和致动脉粥样硬化饮食对小鼠主动脉免疫炎症环境的影响。
DOI：
10.1016/j.bbi.2011.06.007
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Conti,Bruno
中科院分区：
文献类型：
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作者：
Marcondes,MariaCeciliaGaribaldi;Zhukov,Viktor;Bradlow,Henry;Sanchez-Alavez,Manuel;Gonzalez,AlejandroSanchez-Alavez;Curtiss,LindaK;Conti,Bruno
炎症和应激被认为是两个重要的动脉粥样硬化形成因素。由于压力可以影响白细胞分布，我们假设压力介导的白细胞外渗可以改变动脉壁的炎症环境，可能有助于动脉粥样硬化的形成。为了验证这一假设，我们评估了慢性应激3个月和12个月的C57 B1/6小鼠主动脉的炎症环境，并将其与年龄匹配的非应激动物进行比较。实验在喂食常规食物或致动脉粥样硬化饮食的小鼠中进行。两种处理均增加血管和白细胞粘附分子的表达以及白细胞积聚。在3个月时，应激而非致动脉粥样硬化饮食增加了CD 4细胞、CD 8细胞、巨噬细胞、树突状细胞和中性粒细胞的数量。这些变化与ICAM-1和VCAM-1、E-选择素和神经肽Y的转录升高有关。在12个月时，应激或高胆固醇对提高CD 8和巨噬细胞数量的作用相似，并且对研究的所有细胞类型的数量具有协同作用。在这个时间点，还观察到在主动脉中E-选择素和NPY的水平上的强协同作用，但在循环中没有。尽管有这些影响，动脉壁的组织学和形态学改变是严重的致动脉粥样硬化饮食，但不是在应力组。因此，虽然压力和致动脉粥样硬化饮食都可能影响白细胞在主动脉中的积聚，但它们对动脉粥样硬化的作用可能不同。
Inflammation and stress are regarded as two important atherogenic factors. Because stress can affect leukocyte distribution, we hypothesized that stress-mediated leukocyte extravasation can modify the inflammatory environment of the arterial wall possibly contributing to atherogenesis. To test this hypothesis we evaluated the inflammatory environment of the aorta in C57Bl/6 mice subjected to 3 and 12months of chronic stress and compared it to age matched non-stressed animals. Experiments were carried out in mice fed regular chow or atherogenic diets. Both treatments increased the expression of vascular and leukocyte adhesion molecules and leukocyte accumulation. At 3months, stress but not an atherogenic diet elevated the number of CD4 cells, CD8 cells, macrophages, dendritic cells and neutrophils. These changes were associated with elevation of transcripts for ICAM-1 and VCAM-1, E-selectin and neuropeptide Y. At 12months, stress or high cholesterol acted similarly to elevate the number of CD8 and macrophages, and synergistically on the number of all cell types investigated. At this time-point, strong synergism was also observed on the level of E-selectin and NPY in the aorta, but not in the circulation. Despite these effects, histological and morphological alterations of the arterial wall were severe in the atherogenic diet, but not in the stress groups. Thus, although stress and an atherogenic diet may both affect leukocyte accumulation in the aorta, they may contribute differently to atherogenesis.