PNEUMOCYSTIS-CARINII PNEUMONIA IN SIMIAN IMMUNODEFICIENCY VIRUS-INFECTION - IMMUNOHISTOLOGICAL AND SCANNING AND TRANSMISSION ELECTRON-MICROSCOPIC STUDIES
PNEUMOCYSTIS-CARINII PNEUMONIA IN SIMIAN IMMUNODEFICIENCY VIRUS-INFECTION - IMMUNOHISTOLOGICAL AND SCANNING AND TRANSMISSION ELECTRON-MICROSCOPIC STUDIES
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DOI：
10.1002/path.1711640212
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发表时间：
1991-06-01
影响因子：
7.3
通讯作者：
GREENAWAY, PJ
中科院分区：
文献类型：
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作者：
BASKERVILLE, A;DOWSETT, AB;GREENAWAY, PJ
用免疫组化、扫描电镜和透射电镜观察了17只感染猴免疫缺陷病毒的恒河猴中6只发生卡氏肺孢子虫肺炎。 单克隆抗体/链霉抗生物素蛋白-生物素-过氧化物酶染色法对于检测小的早期病变中的微生物具有高度敏感性，并且比传统的银浸渍法更敏感和特异。 对石蜡包埋肺组织进行再处理以进行扫描电子显微镜检查，并使用视频打印机制作光镜病变的摄影蒙太奇，从而可以通过两种方法检查并比较相同区域的组织。 恒河猴体内卡氏肺孢子虫的超微结构形态与人类相同，组织学和电子显微镜病变也相同，包括肺纤维化。 滋养体附着于肺泡I型上皮细胞，主要是通过紧密附着于质膜，但扫描电子显微镜也显示附着于细长的丝状伪足。 感染肺泡中存在少量巨噬细胞，尽管观察到卡氏肺孢子虫滋养体的吞噬作用和消化作用，但其水平似乎很低。
Pneumocystis carinii pneumonia occurred in 6 of 17 rhesus monkeys infected with simian immunodeficiency virus and was studied by immunohistochemistry and by scanning and transmission electron microscopy. A monoclonal antibody/streptavidin-biotin-peroxidase staining method was highly sensitive for detecting the organisms in small, early lesions and was much more sensitive and specific than traditional silver impregnation methods. Reprocessing of paraffin wax-embedded lung tissue for scanning electron microscopy and use of a video printer to produce a photographic montage of light microscopic lesions allowed the same areas of tissue to be examined and compared by both methods. The ultrastructural morphology of P. carinii in the rhesus monkey was identical to that in man, as were the histological and electron microscopic lesions, including pulmonary fibrosis. Trophozoites were seen attached to alveolar type I epithelium mainly by intimate apposition to the plasma membrane, but scanning electron microscopy also showed attachment by elongated filopodia. Few macrophages were present in infected alveoli, and though phagocytosis followed by digestion of P. carinii trophozoites was observed, it appeared to occur at a very low level.