A disadvantageous effect of adsorption of barium by melanin on transforming activity.
A disadvantageous effect of adsorption of barium by melanin on transforming activity.
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DOI：
10.1016/j.chemosphere.2018.07.022
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发表时间：
2018-07
期刊：
影响因子：
8.8
通讯作者：
Yasuhiro Omata;Masafumi Yoshinaga;I. Yajima;N. Ohgami;Kazunori Hashimoto;Kaho Higashimura;Akira Tazaki;Masashi Kato
中科院分区：
文献类型：
--
作者：
Yasuhiro Omata;Masafumi Yoshinaga;I. Yajima;N. Ohgami;Kazunori Hashimoto;Kaho Higashimura;Akira Tazaki;Masashi Kato
目前，已报道了黑色素的有益作用和钡的有害作用。然而，人们对钡的吸附知之甚少，对黑色素吸附钡的生物学意义更是知之甚少。在这项研究中，我们发现，有一个很强的相关性之间的数字化水平的皮肤色素沉着和小鼠皮肤中的钡水平相比，皮肤色素沉着水平和钡（镁，钙和锶）的同源元素的水平之间的相关性。高色素沉着皮肤皮下注射钡浓度高于低色素沉着皮肤。我们的无细胞实验中使用的朗缪尔等温线的钡的合成黑色素的吸附也提供了直接的证据，钡的黑色素的吸附。然后，我们研究了黑色素介导的钡吸附的生物学意义。我们发现，在5 μM钡培养24小时后，在有色素的黑素细胞（melan-a）中钡介导的转化活性增加，而在无色素的黑素细胞（melan-c）中则没有。两者合计，我们的研究结果不仅表明黑色素在小鼠，细胞和无细胞系统中的钡吸附，但也表明黑色素的钡吸附在培养细胞的转化活性的不利影响。
At present, beneficial effects of melanin and harmful effects of barium have been reported. However, little is known about the adsorption of barium, and even less is known about the biological significance of adsorption of barium by melanin. In this study, we showed that there was a strong correlation between the digitalized level of skin pigmentation and barium level in murine skin compared to the correlations between skin pigmentation level and levels of homologous elements of barium (magnesium, calcium and strontium). The concentration of subcutaneously injected barium in skin with a high level of pigmentation was higher than that in skin with a low level of pigmentation. Our cell-free experiment using the Langmuir isotherm for adsorption of barium in synthetic melanin also provided direct evidence of adsorption of barium by melanin. We then investigated the biological significance of melanin-mediated barium adsorption. We found barium-mediated increase in transforming activity in pigmented melanocytes (melan-a) but not in unpigmented melanocytes (melan-c) after confirming that the barium level in melan-a melanocytes was 3.4-fold higher than that in melan-c melanocytes after culture of 5 μM barium for 24 h. Taken together, our results not only indicate adsorption of barium by melanin in mice, cells and cell-free systems but also suggest a disadvantageous effect of adsorption of barium by melanin on transforming activity in cultured cells.