FLOW STIMULATES ENDOTHELIAL-CELLS TO RELEASE A NITROVASODILATOR THAT IS POTENTIATED BY REDUCED THIOL
FLOW STIMULATES ENDOTHELIAL-CELLS TO RELEASE A NITROVASODILATOR THAT IS POTENTIATED BY REDUCED THIOL
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DOI：
10.1152/ajpheart.1990.259.3.h804
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发表时间：
1990-09-01
影响因子：
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通讯作者：
LOSCALZO, J
中科院分区：
文献类型：
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作者：
COOKE, JP;STAMLER, J;LOSCALZO, J
我们设计了一个新的系统来研究流量介导的内皮依赖性血管舒张。将兔胸主动脉血管环固定在充满生理盐水的流动室中，进行等长张力记录。流动室包含搅拌棒，并安装在磁力搅拌器上以诱导皮质流动。去甲肾上腺素（NE，10 ~（-6）M）引起血管环收缩。牛内皮细胞上的微载体珠加入到室中的收缩NE在没有流量的影响不大。血流诱导的血管环内皮依赖性舒张依赖于流速。用亚甲蓝牛血红蛋白或N-单甲基-L-精氨酸使舒张无效或逆转为收缩。相反，N-乙酰-L-半胱氨酸增强了血流介导的舒张。此外，在N-乙酰-L-半胱氨酸的存在下，内皮依赖性舒张因子的半衰期增加。总之，刺激的流动诱导释放的内皮细胞的一个可扩散的，短暂的因素与属性的硝基血管扩张剂。这种内源性血管扩张剂的作用被还原的巯基N-乙酰-L-半胱氨酸增强。
We designed a novel system to study flow-mediated endothelium-dependent vasodilation. Vascular rings of rabbit thoracic aorta were mounted for isometric tension recording in a flow chamber filled with physiological saline solution. The flow chamber contained a stir bar and was mounted on a magnetic stirrer to induce cortical flow. Norepinephrine (NE, 10-6 M) induced contraction of the vascular rings. Bovine endothelial cells on microcarrier beads added to the chamber had little effect on contraction to NE in the absence of flow. Flow induced endothelium-dependent relaxation of the vascular rings that was dependent on the flow rate. Relaxations were annulled or reversed to a contraction with methylene blue bovine hemoglobin, or N-monomethyl-L-arginine. Conversely, N-acetyl-L-cysteine augmented the flow-mediated relaxation. Furthermore, in the presence of N-acetyl-L-cysteine, the half-life of the endothelium-dependent relaxing factor was increased. In conclusion, the stimulus of flow induces the release by endothelial cells of a diffusible, short-lived factor with the attributes of a nitrovasodilator. The action of this endogenous vasodilator is augmented by the reduced thiol N-acetyl-L-cysteine.