Assessment of cytotoxicity using electric cell-substrate impedance sensing: Concentration and time response function approach
Assessment of cytotoxicity using electric cell-substrate impedance sensing: Concentration and time response function approach
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DOI：
10.1021/ac025848f
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发表时间：
2002-11-15
影响因子：
7.4
通讯作者：
Luong, JHT
中科院分区：
文献类型：
--
作者：
Xiao, CD;Lachance, B;Luong, JHT
本文介绍了一种简单方便的方法，通过电-基底阻抗传感来测量暴露于有毒物质的细胞的浓度和时间响应函数f （C,t）。在预先包被纤维连接蛋白的小金电极上培养成纤维细胞V79，检测其附着和扩散的电阻变化。该方法通过观察附着细胞在抑制剂作用下的反应功能，可以很容易地筛选化学细胞毒性。通过测定三种试验模型（氯化镉、砷酸钠和苯扎氯铵）的抑制百分比与抑制剂浓度的函数关系，对三种试验模型的细胞毒性进行了量化。根据响应函数得出的半抑制浓度，即达到50%抑制所需的浓度，与使用标准中性红测定法得到的结果吻合良好。
This paper describes a simple and convenient method to measure the concentration and time response function f (C,t) of cells exposed to a toxicant by electric cell-substrate impedance sensing. Attachment and spreading of fibroblastic V79 cells cultured on small gold electrodes precoated with fibronectin were detected as electrical resistance changes. With this method, chemical cytotoxicity was easily screened by observing the response function of attached cells in the presence of inhibitor. The cytotoxicities of three test models, cadmium chloride, sodium arsenate, and benzalkonium chloride, were quantified by measuring the percentage inhibition as a function of the inhibitor concentration. The half-inhibition concentration, the required concentration to achieve 50% inhibition, derived from the response function agreed well with the results obtained using the standard neutral red assay.