Improvement of Uveal and Capsular Biocompatibility of Hydrophobic Acrylic Intraocular Lens by Surface Grafting with 2-Methacryloyloxyethyl Phosphorylcholine-Methacrylic Acid Copolymer.
Improvement of Uveal and Capsular Biocompatibility of Hydrophobic Acrylic Intraocular Lens by Surface Grafting with 2-Methacryloyloxyethyl Phosphorylcholine-Methacrylic Acid Copolymer.
复制标题
2-甲基丙烯酰氧基乙基磷酰胆碱-甲基丙烯酸共聚物表面接枝改善疏水性丙烯酸人工晶状体葡萄膜和囊膜生物相容性
DOI：
10.1038/srep40462
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发表时间：
2017-01-13
影响因子：
4.6
通讯作者：
Ren L
中科院分区：
文献类型：
--
作者：
Tan X;Zhan J;Zhu Y;Cao J;Wang L;Liu S;Wang Y;Liu Z;Qin Y;Wu M;Liu Y;Ren L
人工晶状体（IOL）的生物相容性对于白内障术后视力重建至关重要。可折叠疏水性丙烯酸人工晶状体易受细胞外基质蛋白和细胞粘附的影响，导致术后炎症和囊膜浑浊的发生率增加。为了提高IOL的生物相容性，我们合成了亲水性共聚物P(MPC-MAA)，并通过空气等离子体处理将该共聚物接枝到IOL表面。 X射线光电子能谱、原子力显微镜和静态水接触角分别用于表征IOL表面的化学变化、形貌和亲水性。耗散石英晶体微天平（QCM-D）表明 P(MPC-MAA) 修饰的 IOL 具有抗蛋白质吸附的能力。此外，P(MPC-MAA)修饰在体外抑制晶状体上皮细胞(LEC)的粘附和增殖。为了分析体内葡萄膜和囊膜的生物相容性，我们在超声乳化术后将 P(MPC-MAA) 修饰的人工晶状体植入兔子体内。 P（MPC-MAA）修饰显着减少术后炎症和前囊混浊（ACO），并且不影响后囊混浊（PCO）。总的来说，我们的研究表明，P(MPC-MAA) 的表面修饰可以显着改善疏水性丙烯酸 IOL 的葡萄膜和囊膜生物相容性，这可能有利于血水屏障损伤的患者。
Biocompatibility of intraocular lens (IOL) is critical to vision reconstruction after cataract surgery. Foldable hydrophobic acrylic IOL is vulnerable to the adhesion of extracellular matrix proteins and cells, leading to increased incidence of postoperative inflammation and capsule opacification. To increase IOL biocompatibility, we synthesized a hydrophilic copolymer P(MPC-MAA) and grafted the copolymer onto the surface of IOL through air plasma treatment. X-ray photoelectron spectroscopy, atomic force microscopy and static water contact angle were used to characterize chemical changes, topography and hydrophilicity of the IOL surface, respectively. Quartz crystal microbalance with dissipation (QCM-D) showed that P(MPC-MAA) modified IOLs were resistant to protein adsorption. Moreover, P(MPC-MAA) modification inhibited adhesion and proliferation of lens epithelial cells (LECs)in vitro. To analyze uveal and capsular biocompatibilityin vivo, we implanted the P(MPC-MAA) modified IOLs into rabbits after phacoemulsification. P(MPC-MAA) modification significantly reduced postoperative inflammation and anterior capsule opacification (ACO), and did not affect posterior capsule opacification (PCO). Collectively, our study suggests that surface modification by P(MPC-MAA) can significantly improve uveal and capsular biocompatibility of hydrophobic acrylic IOL, which could potentially benefit patients with blood-aqueous barrier damage.