Enhanced xylose fermentation capacity related to an altered glucose sensing and repression network in a recombinant Saccharomyces cerevisiae
Enhanced xylose fermentation capacity related to an altered glucose sensing and repression network in a recombinant Saccharomyces cerevisiae
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DOI：
10.4161/bioe.25542
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发表时间：
2013-06
期刊：
影响因子：
4.9
通讯作者：
Yu Shen;J. Hou;X. Bao
中科院分区：
文献类型：
--
作者：
Yu Shen;J. Hou;X. Bao
葡萄糖和木糖的共发酵是降低由木质纤维素材料生产的生物燃料或化学品的价格的问题之一。通过合理的遗传操作，获得了一株葡萄糖和木糖共利用酿酒酵母。在木糖中进行了非理性进化，工程菌的木糖利用效率显著提高。转录组的研究结果表明，Snf 1/Mig 1介导的调控，葡萄糖传感和阻遏网络的一部分，在进化菌株中发生了改变，可能与木糖利用的增强。
The co-fermentation of glucose and xylose is one of the issues in decreasing the price of biofuel or chemicals produced from lignocellulosic materials. A glucose and xylose co-utilizing Saccharomyces cerevisiae was obtained through rational genetic manipulation. Non-rational evolution in xylose was performed, and the xylose utilization efficiency of the engineered strain was significantly enhanced. The results of transcriptome study suggested that Snf1/Mig1-mediated regulation, a part of glucose sensing and repression network, was altered in the evolved strain and might be related to the enhancement of xylose utilization.