Increased migration in late G1 phase in cultured smooth muscle cells
Increased migration in late G1 phase in cultured smooth muscle cells
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DOI：
10.1152/ajpcell.2000.279.4.c999
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发表时间：
2000-10-01
影响因子：
5.5
通讯作者：
Ohsawa, N
中科院分区：
文献类型：
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作者：
Fukui, R;Amakawa, M;Ohsawa, N
动脉损伤后，平滑肌细胞(SMC)的迁移和增殖参与了新生内膜的形成。然而，这些细胞的迁移和增殖之间的关系尚不清楚。为了区分迁移和增殖，我们采用了细胞周期不同阶段的SMC迁移分析。通过加入不同时间的血清，使去血清的SMC在细胞周期的不同阶段同步化。血小板衍生生长因子B链同源二聚体诱导的SMC迁移作用最强，主要发生在G(1)(G(1b))晚期。此外，在非同步SMC中，已迁移的SMC中有65%-75%处于G1b期。与其他细胞周期相比，G(1b)期SMC的磷酸化肌球蛋白轻链主要集中在细胞周边。有趣的是，在富含G(1b)的SMC中，肌球蛋白的Triton X-100不溶部分显著减少。这些结果表明，SMC的迁移活动可能与G(1b)期相耦合。肌球蛋白的磷酸化和滞留可能解释了导致迁移增加的一些特性。
Migration and proliferation of smooth muscle cells (SMC) contribute to neointimal formation after arterial injury. However, the relation between migration and proliferation in these cells is obscure. To discriminate between migration and proliferation, we employed a migration assay of SMC at different phases of the cell cycle. Serum-deprived SMC were synchronized in different phases of the cell cycle by addition of serum for various periods of time. Migration induced by platelet-derived growth factor B-chain homodimer was maximal in SMC that were predominantly in the late G(1) (G(1b)) phase. In addition, in nonsynchronized SMC, 65-75% of SMC that had migrated were in the G1b phase. Phosphorylated myosin light chain was enriched around the cell periphery in SMC in the G(1b) phase compared with SMC in the other cell cycle phases. Interestingly, the Triton X-100-insoluble fraction of myosin was remarkably decreased in G(1b)-enriched SMC. These findings suggest that migratory activity of SMC may be coupled with the G(1b) phase. The phosphorylation and retention of myosin might explain some of the properties responsible for increased migration.