Sensitive Detection of 2,4,6-Trinitrotoluene by Surface Plasmon Fluorescence Spectroscopy
Sensitive Detection of 2,4,6-Trinitrotoluene by Surface Plasmon Fluorescence Spectroscopy
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表面等离子体荧光光谱法灵敏检测 2,4,6-三硝基甲苯
DOI：
10.1109/icsens.2014.6984999
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Takeshi Onodera and Kiyoshi Toko
中科院分区：
文献类型：
--
作者：
Satoshi Ito;Shuhei Tanaka;Rui Yatabe;Takeshi Onodera and Kiyoshi Toko
我们研制了一套紧凑的表面等离子体荧光光谱(SPFS)测量系统和检测2，4，6-三硝基甲苯(TNT)的传感器表面。在光学系统中，我们采用半导体激光器作为激发荧光的光源。然后，使用扩束器将扇形光束从激光二极管照射到Au薄膜上。用光电倍增管探测器捕获荧光发射。通过自组装单分子膜对传感器表面进行TNT类似物修饰。用荧光标记的抗TNT单抗在SPFS体系中通过间接竞争分析获得了TNT在ppt范围内的检测限。
We developed a compact measurement system for surface plasmon fluorescence spectroscopy (SPFS) and a sensor surface for the detection of 2,4,6-trinitrotoluene (TNT). We employed a laser diode as a light source in the optical system for exciting fluorescence. Then, a fan-shaped beam was irradiated from the laser diode to a Au thin film using a beam expander. Fluorescence emission was captured using a photomultiplier detector. The sensor surface was modified with TNT analogues via a self-assembled monolayer. A limit of detection in the ppt range for TNT was obtained using a fluorescence-labeled anti-TNT monoclonal antibody in the SPFS system by indirect competitive assay.