Protective paracrine effect of mesenchymal stem cells on cardiomyocytes
Protective paracrine effect of mesenchymal stem cells on cardiomyocytes
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间充质干细胞对心肌细胞的旁分泌保护作用
DOI：
10.1631/jzus.b0920153
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发表时间：
2009-07
期刊：
影响因子：
--
通讯作者：
Ai-na HE
中科院分区：
文献类型：
--
作者：
Mei-xiang XIANG;Jian-an WANG;Chun GUI;Ai-na HE
目的检测骨髓间充质干细胞(MSCs)对体外培养心肌细胞的保护作用，探讨其抗细胞凋亡的信号转导途径。骨髓间充质干细胞在无血清Dulbecco‘s改良Eagle培养液(DMEM)中缺氧培养。培养的新生SD大鼠心肌细胞暴露于低氧/复氧(H/R)环境中，并用MSC培养液处理。用Annexin-V-异硫氰酸荧光素、Hoechst 33342和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法对心肌细胞进行染色。用荧光显微镜检测心肌细胞线粒体膜电位。免疫印迹法检测心肌细胞凋亡诱导因子(AIF)、凋亡诱导因子(AIF)和半胱氨酸天冬氨酸氨基转移酶-3(caspase-3)的表达。结果MSC培养液可减少H/R诱导的心肌细胞凋亡，增加Bcl2/Bax比值，减少细胞色素C和AIF从线粒体释放到胞浆。结论MSCs通过旁分泌途径保护心肌细胞免受H/R诱导的心肌细胞凋亡。
ObjectiveThe aim of this study was to test the protective effect of mesenchymal stem cells (MSCs) on cardiomyocytes in vitro and to investigate the anti-apoptotic signaling pathway.MethodsMSCs from Sprague-Dawley (SD) rats were separated and cultured. MSC medium was collected from MSCs cultured in serum-free Dulbecco’s modified eagle medium (DMEM) under hypoxia. Cultured cardiomyocytes from neonatal SD rats were exposed to hypoxia/reoxygenation (H/R) and treated with MSC medium. The apoptotic cardiomyocytes were stained with Annexin-V-fluorescein isothiocyanate (FITC), Hoechst 33342 and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL). The mitochondrial transmembrane potential of cardiomyocytes was assessed using a fluorescence microscope. The expression of Bcl-2, Bax, cytochrome C, apoptosis-induced factor (AIF), and caspase-3 was tested by Western blot analysis.ResultsOur data demonstrated that MSC medium reduced H/R-induced cardiomyocyte apoptosis, increased the Bcl-2/Bax ratio, and reduced the release of cytochrome C and AIF from mitochondria into the cytosol.ConclusionMSCs protected the cardiomyocytes from H/R-induced apoptosis through a mitochondrial pathway in a paracrine manner.
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影响因子：
168.9
作者：
Janssens, S;Dubois, C;Van de Werf, F
通讯作者：
Van de Werf, F
影响因子：
82.9
作者：
Kocher, AA;Schuster, MD;Itescu, S
通讯作者：
Itescu, S
DOI：
10.1080/00365510801918761
发表时间：
2008-01-01
影响因子：
2.1
作者：
Chen, T. -L.;Wang, J. -A.;Cao, J.
通讯作者：
Cao, J.
影响因子：
64.8
作者：
Susin, SA;Lorenzo, HK;Kroemer, G
通讯作者：
Kroemer, G
影响因子：
20.1
作者：
Uemura, Ryota;Xu, Meifeng;Ashraf, Muhammad
通讯作者：
Ashraf, Muhammad