Pruriceptive spinothalamic tract neurons: physiological properties and projection targets in the primate
Pruriceptive spinothalamic tract neurons: physiological properties and projection targets in the primate
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DOI：
10.1152/jn.00206.2012
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发表时间：
2012-09-01
影响因子：
2.5
通讯作者：
Giesler, Glenn J., Jr.
中科院分区：
文献类型：
--
作者：
Davidson, Steve;Zhang, Xijing;Giesler, Glenn J., Jr.
引用本文：david S， Zhang X, Khasabov SG, Moser HR, Honda CN, Simone DA, Giesler GJ Jr.灵长类动物感受性脊髓丘脑束神经元的生理特性和投射目标。[J] .中国生物医学工程学报，2016,31(4):557 - 557。首次发表于2012年6月20日；doi: 10.1152 / jn.00206.2012。-外周起源的瘙痒需要信息从脊髓传递到大脑以感知。本研究采用活体电生理学方法对灵长类动物腰椎脊髓丘脑束（STT）神经元进行了功能表征，以确定这些细胞在感知信息传递中的作用。在对每个细胞的皮肤感受野依次施加组胺和cowhage（一种非组胺性止痒剂）的同时，通过反向刺激鉴定出111个STT神经元，并记录下来。20%的STT神经元对组胺有反应，13%对奶牛有反应，2%对两者都有反应。所有感觉性STT神经元都是机械敏感的，并且对热、皮内辣椒素或两者都有反应。与位于深背角的STT神经元相比，位于浅背角的STT神经元对瘙痒素的反应更大，持续时间更长。痛觉性STT神经元在痛觉原和辣椒素的激活下以爆裂模式放电。微反位图用于确定丘脑内感受性STT轴突的终止区。来自组胺反应性和奶牛反应性STT神经元的轴突终止于几个丘脑核，包括腹侧后外侧核、腹侧后下核和后核。此外，还发现奶牛反应神经元的轴突终止于扣上核和膝状核内侧。组胺反应性STT神经元在组胺反应后对感受野的温和抚摸敏感，这表明脊髓机制的异变。结果表明，灵长类动物的感知信息由多模态STT神经元以组胺能或非组胺能途径编码，并传递到腹底复合体和后丘脑。
Davidson S, Zhang X, Khasabov SG, Moser HR, Honda CN, Simone DA, Giesler GJ Jr. Pruriceptive spinothalamic tract neurons: physiological properties and projection targets in the primate. J Neurophysiol 108: 1711-1723, 2012. First published June 20, 2012; doi:10.1152/jn.00206.2012.-Itch of peripheral origin requires information transfer from the spinal cord to the brain for perception. Here, primate spinothalamic tract (STT) neurons from lumbar spinal cord were functionally characterized by in vivo electrophysiology to determine the role of these cells in the transmission of pruriceptive information. One hundred eleven STT neurons were identified by antidromic stimulation and then recorded while histamine and cowhage )a nonhistaminergic pruritogen) were sequentially applied to the cutaneous receptive field of each cell. Twenty percent of STT neurons responded to histamine, 13% responded to cowhage, and 2% responded to both. All pruriceptive STT neurons were mechanically sensitive and additionally responded to heat, intradermal capsaicin, or both. STT neurons located in the superficial dorsal horn responded with greater discharge and longer duration to pruritogens than STT neurons located in the deep dorsal horn. Pruriceptive STT neurons discharged in a bursting pattern in response to the activating pruritogen and to capsaicin. Microantidromic mapping was used to determine the zone of termination for pruriceptive STT axons within the thalamus. Axons from histamine-responsive and cowhage-responsive STT neurons terminated in several thalamic nuclei including the ventral posterior lateral, ventral posterior inferior, and posterior nuclei. Axons from cowhage-responsive neurons were additionally found to terminate in the suprageniculate and medial geniculate nuclei. Histamine-responsive STT neurons were sensitized to gentle stroking of the receptive field after the response to histamine, suggesting a spinal mechanism for alloknesis. The results show that pruriceptive information is encoded by polymodal STT neurons in histaminergic or nonhistaminergic pathways and transmitted to the ventrobasal complex and posterior thalamus in primates.