Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes.
Three-dimensional fluorescent microscopy via simultaneous illumination and detection at multiple planes.
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DOI：
10.1038/srep31445
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发表时间：
2016-08-16
影响因子：
4.6
通讯作者：
Liu Z
中科院分区：
文献类型：
--
作者：
Ma Q;Khademhosseinieh B;Huang E;Qian H;Bakowski MA;Troemel ER;Liu Z
传统的光学显微镜本质上是一种二维（2D）成像工具。物镜透镜、目镜和图像传感器都被设计为捕获从2D“物平面”发射的光。现有的技术，如共聚焦或光片荧光显微镜必须利用机械扫描，时间复用过程，以捕获3D图像。本文提出了一种基于多焦平面同时照明和检测的三维光学显微方法。这通过添加两个衍射光学元件来修改照明和检测光学器件来实现。我们证明，这种技术的图像质量是传统的光片荧光显微镜的优点，同时成像的多个轴向平面和减少的扫描次数所需的图像的整个样品体积。
The conventional optical microscope is an inherently two-dimensional (2D) imaging tool. The objective lens, eyepiece and image sensor are all designed to capture light emitted from a 2D ‘object plane’. Existing technologies, such as confocal or light sheet fluorescence microscopy have to utilize mechanical scanning, a time-multiplexing process, to capture a 3D image. In this paper, we present a 3D optical microscopy method based upon simultaneously illuminating and detecting multiple focal planes. This is implemented by adding two diffractive optical elements to modify the illumination and detection optics. We demonstrate that the image quality of this technique is comparable to conventional light sheet fluorescent microscopy with the advantage of the simultaneous imaging of multiple axial planes and reduced number of scans required to image the whole sample volume.
影响因子：
9.2
作者：
Frady, E. Paxon;Palmer, Christopher R.;Kristan, William B., Jr.
通讯作者：
Kristan, William B., Jr.