Dietary omega-3 fatty acid does not improve male infertility caused by lysophospholipid acyltransferase 3 (LPLAT3/AGPAT3) deficiency
Dietary omega-3 fatty acid does not improve male infertility caused by lysophospholipid acyltransferase 3 (LPLAT3/AGPAT3) deficiency
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膳食 omega-3 脂肪酸不能改善溶血磷脂酰基转移酶 3 (LPLAT3/AGPAT3) 缺乏引起的男性不育症
DOI：
10.1016/j.bbrc.2023.04.043
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发表时间：
2023
影响因子：
3.1
通讯作者：
Shindou Hideo
中科院分区：
文献类型：
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作者：
Nagata Katsuyuki;Kakizaki Yuusuke;Yanagida Keisuke;Arai Tetsuya;Nakano Kenta;Hamano Fumie;Goto Motohito;Okamura Tadashi;Shimizu Takao;Shindou Hideo
二十二碳六烯酸(DHA)是一种omega-3脂肪酸，通常以磷脂的形式存在于细胞膜上。溶血磷脂酰基转移酶3(LPLAT3；AGPAT3)是将DHA结合到磷脂中的主要酶。LPLAT3-KO小鼠表现出雄性不育和视觉功能障碍，并分别伴有睾丸和视网膜中含有DHA的磷脂(PL-DHA)减少。在这项研究中，我们评估了主要由三酰甘油结合的DHA(鱼油)和PL结合的DHA(鲑鱼籽油)组成的日粮对广泛组织中PL-DHA含量和生殖功能的影响。这两种饮食都能增加野生型(WT)小鼠大多数组织中含有磷脂酰胆碱(PC)的DHA。尽管LPLAT3-KO小鼠通过食用这两种饮食中的任何一种，在睾丸和精子中获得了最少量的PC-DHA，但生殖功能并没有改善。本研究表明，富含DHA的饮食不能恢复足够的PL-DHA来改善LPLAT3-KO小鼠的男性不育。或者，PL-DHA可以由LPLAT3生物合成，但不能通过外部补充合成，这可能是正常生殖功能所必需的。
Docosahexaenoic acid (DHA), an omega-3 fatty acid, usually presents as a constituent of phospholipids in the cellular membrane. Lysophospholipid acyltransferase 3 (LPLAT3; AGPAT3) is the primary enzyme that incorporates DHA into phospholipids. LPLAT3-KO mice show male infertility and visual dysfunction accompanied by decreased phospholipids (PLs) containing DHA (PL-DHA) in the testis and retina, respectively. In this study, we evaluated the effect of diets consisting mainly of triacylglycerol-bound DHA (fish oil) and PL-bound DHA (salmon roe oil) on the amount of PL-DHA in a broad range of tissues and on reproductive functions. Both diets elevated phosphatidylcholines (PCs)-containing DHA in most tissues of wild type (WT) mice. Although LPLAT3-KO mice acquired a minimal amount of PC-DHA in the testes and sperm by eating either of the diets, reproductive function did not improve. The present study suggests that DHA-rich diets do not restore sufficient PL-DHA to improve male infertility in LPLAT3-KO mice. Alternatively, PL-DHA can be biosynthesized by LPLAT3 but not by external supplementation, which may be necessary for normal reproductive function.