HPLC-PDA-MS fingerprinting to assess the authenticity of pomegranate beverages

HPLC-PDA-MS fingerprinting to assess the authenticity of pomegranate beverages
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DOI:
10.1016/j.foodchem.2012.05.108
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发表时间:
2012-12-01
期刊:
影响因子:
8.8
通讯作者:
Crozier, Alan
Crozier, Alan
中科院分区:
农林科学1区
文献类型:
--
作者:
Borges, Gina;Crozier, Alan

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使用荧光,PDA和质谱检测的HPLC来分析波尔多红酒的(多)苯酚含量,POM奇妙的石榴汁以及其他三种饮料,这些饮料是由100%石榴汁制成的。红酒和POM奇妙的果汁包含特征性花青素谱,后者的特征也具有Ellagitannins的存在,而前者则是Flavan-3-ols单体和procyanidin二聚体和三聚体。其他三种石榴产品都含有预期的ellagitannins,但它们的花青素曲线是红酒和石榴花青素的混合物。它们还包含Flavan-3-ol单体和procyanidin二聚体和三聚体,通常在100%石榴饮料中检测到成分。因此,基于HPLC的过程提供了一种直接的方法,可以使用源自红色葡萄的成分,访问基于石榴的产品的真实性。 (c)2012 Elsevier Ltd.保留所有权利。
HPLC with fluorescence, PDA and mass spectrometric detection were used to analyse the (poly)phenol content of a Bordeaux red wine, POM Wonderful pomegranate juice and three other beverages advertised as being made from 100% pomegranate juice. The red wine and POM Wonderful juice contained characteristic anthocyanin profiles with the latter also being characterised by the presence of ellagitannins and the former by flavan-3-ols monomers and procyanidin dimers and trimers. The three other pomegranate products all contained the expected ellagitannins but their anthocyanin profiles were a mixture of red wine and pomegranate anthocyanins. They also contained flavan-3-ol monomers and procyanidin dimers and trimers, components not usually detected in 100% pomegranate beverages. The HPLC-based procedures, therefore, provide a straight-forward means of accessing the authenticity of pomegranate-based products with the ready detection of constituents derived from red grapes. (C) 2012 Elsevier Ltd. All rights reserved.