Ginkgo Biloba Extract Attenuates Oxidative Stress and Apoptosis in Mouse Cochlear Neural Stem Cells
Ginkgo Biloba Extract Attenuates Oxidative Stress and Apoptosis in Mouse Cochlear Neural Stem Cells
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DOI：
10.1002/ptr.5572
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发表时间：
2016-05-01
影响因子：
7.2
通讯作者：
Wang, Bin
中科院分区：
文献类型：
--
作者：
Wang, Congpin;Wang, Bin
在器官或Corti中，氧化应激可导致听力损伤，而神经干细胞（NSCs）在治疗听力损失方面具有巨大的治疗潜力。银杏叶提取物（GBE）在治疗神经损伤和疾病中具有抗氧化和抗凋亡作用。以过氧化氢诱导的小鼠耳蜗神经干细胞氧化应激为模型，研究银杏叶提取物的抗氧化作用。发现GBE处理通过显著减弱过氧化氢诱导的氧化应激而显著促进NSC的活力。此外，GBE的这种抗氧化功能也能够防止线粒体去极化和随后的凋亡。此外，GBE的抗凋亡作用是通过拮抗内在线粒体凋亡途径介导的，其中GBE可以逆转关键内在凋亡途径因子包括Bcl-2、Bax和Caspase-3的变化。我们的数据提供了关于GBE通过减弱氧化应激触发的内在凋亡在保护耳蜗神经干细胞中的有益作用的第一份报告，因此支持GBE在预防氧化应激相关听力损失中的潜在治疗价值。版权所有（c）2016约翰威利父子有限公司
In the organ or Corti, oxidative stress could result in damage to the hearing, and neural stem cells (NSCs) hold great therapeutic potential in treating hearing loss. Ginkgo biloba extract (GBE) has been widely shown to exhibit anti-oxidative and anti-apoptotic effects in treatments of neural damage and disorder. Using hydrogen peroxide to induced oxidative stress as a model, we investigated the anti-oxidative role of GBE in isolated mouse cochlear NSCs. GBE treatment was found to significantly promote viability of NSCs, by markedly attenuating hydrogen peroxide induced oxidative stress. In addition, this anti-oxidative function of GBE was also able to prevent mitochondrial depolarization and subsequent apoptosis. Moreover, the anti-apoptotic role of GBE was mediated by antagonizing the intrinsic mitochondrial apoptotic pathway, where GBE could reverse the changes in key intrinsic apoptosis pathway factors including Bcl-2, Bax, and Caspase-3. Our data provided the first report on the beneficial role of GBE in protecting cochlear NSCs, by attenuating oxidative stress triggered intrinsic apoptosis, therefore supporting the potential therapeutic value of GBE in preventing oxidative stress-related hearing loss. Copyright (c) 2016 John Wiley & Sons, Ltd.