Ovarian function and early embryo development in immature rats given a superovulatory dose of PMSG, later neutralized by antiserum.
Ovarian function and early embryo development in immature rats given a superovulatory dose of PMSG, later neutralized by antiserum.
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给予超排卵剂量的 PMSG（随后用抗血清中和）的未成熟大鼠的卵巢功能和早期胚胎发育。
DOI：
10.1095/biolreprod25.2.272
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发表时间：
1981
影响因子：
3.6
通讯作者：
D. Armstrong
中科院分区：
文献类型：
--
作者：
E. Walton;D. Armstrong
使用外源性促性腺激素诱导排卵可能与过度卵泡刺激导致的生育力降低有关。为了进一步研究这个问题，在实验（SOV动物）第-2天（参见文本脚注4）的0800-0900小时向29日龄大鼠施用超排卵剂量的40 lU PMSG。对照大鼠接受 4 IU PMSG，该剂量可诱导生理数量的排卵，然后正常怀孕。腹膜内注射已知完全抑制卵巢和子宫重量增加能力的抗血清(a/s)体积40 IU PMSG。第 0 天 1800 小时至 SOy 组（SOV a/s 动物）的 50%。然后将雌性与成熟的可育雄性一起关在笼子里过夜，在第 1 天检查交配证据，并在第 1-5 天 1030-1230 小时处死。收集组织和血液用于类固醇分析，并冲洗输卵管和子宫以确定胚胎的数量和位置。与 SOV 动物相比，接受 a/s 的动物在第 4 天和第 5 天的胚胎恢复率更高。第 5 天，SOV a/s 动物平均回收 10.5 个胚胎，而对照大鼠为 8.2 个，SOy 动物为 2.4 个。在第 5 天，大多数 SOV 动物没有胚胎，而随后所有动物都怀孕了，并且大多数胚胎都在子宫中。在对照大鼠中，所有胚胎在第 5 天都在子宫中。与对照相比，SOy 动物的血清和卵巢孕酮水平高出 3-4 倍，但在施用 a/s 后没有变化，而在施用 a/s 18 小时内，血清和卵巢中的雌二醇-17i3 急剧降低至接近对照大鼠中观察到的水平。这些结果表明，超排卵后的早期胚胎丢失可能至少部分是由于雌激素分泌过多造成的，雌激素分泌可能来自剩余的卵泡。这种损失可能是由于输卵管或子宫环境或两者的过度刺激而发生的。
Use of exogenous gonadotropin to induce ovulation may be associated with reduced fertility resulting from excessive follicular stimulation. To investigate this problem further a superovulatory dose of 40 lU PMSG was administered to 29-day-old rats at 0800-0900 h on Day -2 (see text footnote 4) of the experiment (SOV animals). Control rats received 4 lU PMSG, a dose which induces a physiological number of ovulations followed by a normal pregnancy. A volume of antiserum (a/s) known to inhibit completely the ovarian and uterine weight increasing capacity of 40 IU PMSG was injected i.p. at 1800 h on Day 0 to 50% of the SOy group (SOV a/s animals). Females were then caged with mature fertile males overnight, examined for evidence of mating on Day 1, and sacrificed at 1030-1230 h on Days 1-5. Tissue and blood were collected for steroid analyses, and oviducts and uteri were flushed to determine the number and location of embryos. Animals receiving a/s had higher embryo recovery on Days 4 and 5 compared with SOV animals. On Day 5 a mean of 10.5 embryos was recovered from SOV a/s animals compared with 8.2 in control rats and 2.4 in SOy animals. On Day 5 most SOV animals had no embryos whereas following a/s all animals were pregnant and the majority of embryos were in the uterus. In control rats all embryos were in the uterus on Day 5. Serum and ovarian progesterone levels were 3-4-fold higher in SOy animals compared with controls, but were unchanged by administration of a/s, whereas estradiol-17i3 in both serum and ovaries was drastically reduced to levels close to those observed in control rats within 18 h of administration of a/s. These results suggest that early embryo loss after superovulation may result, at least in part, from excessive estrogen Secretion, probably arising from remaining follicles. This loss could occur through excessive stimulation of either the oviductal or uterine environments or both.