Histopathological correlation of cartilage swelling detected by magnetic resonance imaging in early experimental osteoarthritis
Histopathological correlation of cartilage swelling detected by magnetic resonance imaging in early experimental osteoarthritis
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DOI：
10.1016/j.joca.2004.07.007
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发表时间：
2004-11-01
影响因子：
7
通讯作者：
Herrero-Beaumont, G
中科院分区：
文献类型：
--
作者：
Calvo, E;Palacios, I;Herrero-Beaumont, G
目的：我们以前报道过，软骨厚度的增加是实验性骨关节炎（OA）早期磁共振成像（MRI）最早可测量的变化。我们目前的目标是研究这一发现的显微镜翻译，以了解软骨厚度增量是否代表最早的结构损伤，或者它是否构成一个非渐进的可逆phenomenon.Methods：CA诱导的部分内侧artericectomy在兔。正常和假手术动物用作对照。术后0、2、4、6、8、10和52周，依次评估大体和显微镜下软骨变化，并与MRI结果进行比较。结果：MRI检测到的软骨肿胀与基质蛋白多糖和细胞丢失的耗竭相关，这些与OA的早期进展密切相关。结论：MRI显示的软骨肿胀是由于蛋白多糖耗竭引起的，是OA最早的异常。因为它伴随着细胞损失，它不能仅仅归因于手术创伤，而是代表真正的组织损伤。应仔细评估MRI检测到的体积变化的生物学意义，同时考虑到疾病阶段，因为软骨高度的增加也反映了软骨损伤，而不是修复过程。(C)2004年由Elsevier Ltd代表国际骨关节炎研究协会出版。
Objective: We previously reported that an increase of cartilage thickness is the earliest measurable change by magnetic resonance imaging (MRI) in early stages of experimental osteoarthritis (OA). Our present objective was to study the microscopic translation of this finding in order to know whether the cartilage thickness increment represents the earliest structural damage or whether it alternatively constitutes a non-progressive reversible phenomenon.Methods: CA was induced by partial medial meniscectomy in rabbits. Normal and sham-operated animals were used as controls. Gross and microscopic cartilage changes were sequentially assessed after surgery at 0, 2, 4, 6, 8, 10 and 52 weeks, and compared to MRI findings.Results: The swelling of cartilage detected by MRI correlated with depletion in matrix proteoglycans and cellular loss, which were closely related to the progression of OA at the earliest stages. Abnormalities of the cartilage structure appeared only in advanced OA.Conclusion: Cartilage swelling detected by MRI is due to proteoglycan depletion and represents the earliest abnormality in OA. Because it is accompanied by cellular loss, it cannot be merely attributed to surgical trauma and represents true tissue damage. The biological meaning of volume variations detected by MRI should be assessed carefully taking into account the disease stage as an increase in cartilage height also reflects cartilage damage and not a reparative process. (C) 2004 Published by Elsevier Ltd on behalf of OsteoArthritis Research Society International.