Cytomegalovirus infection drives adaptive epigenetic diversification of NK cells with altered signaling and effector function.
Cytomegalovirus infection drives adaptive epigenetic diversification of NK cells with altered signaling and effector function.
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DOI：
10.1016/j.immuni.2015.02.008
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发表时间：
2015-03-17
期刊：
影响因子：
32.4
通讯作者：
Bryceson YT
中科院分区：
文献类型：
--
作者：
Schlums H;Cichocki F;Tesi B;Theorell J;Beziat V;Holmes TD;Han H;Chiang SC;Foley B;Mattsson K;Larsson S;Schaffer M;Malmberg KJ;Ljunggren HG;Miller JS;Bryceson YT
The mechanisms underlying human natural killer (NK) cell phenotypic and functional heterogeneity are unknown. Here, we describe the emergence of diverse subsets of human NK cells selectively lacking expression of signaling proteins after human cytomegalovirus (HCMV) infection. The absence of B and myeloid cell-related signaling protein expression in these NK cell subsets correlated with promoter DNA hyperme-thylation. Genome-wide DNA methylation patterns were strikingly similar between HCMV-associated adaptive NK cells and cytotoxic effector T cells but differed from those of canonical NK cells. Functional interrogation demonstrated altered cytokine responsiveness in adaptive NK cells that was linked to reduced expression of the transcription factor PLZF. Furthermore, subsets of adaptive NK cells demonstrated significantly reduced functional responses to activated autologous T cells. The present results uncover a spectrum of epigenetically unique adaptive NK cell subsets that diversify in response to viral infection and have distinct functional capabilities compared to canonical NK cell subsets.
