CCK2 (CCKB/gastrin) receptor mediates rapid protein kinase D (PKD) activation through a protein kinase C-dependent pathway
CCK2 (CCKB/gastrin) receptor mediates rapid protein kinase D (PKD) activation through a protein kinase C-dependent pathway
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DOI：
10.1016/s0014-5793(01)02076-2
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发表时间：
2001-01-26
期刊：
影响因子：
3.5
通讯作者：
Rozengurt, E
中科院分区：
文献类型：
--
作者：
Chiu, T;Rozengurt, E
在稳定转染CCK2 （CCKB/胃泌素）受体的大鼠-1细胞中加入胃泌素或胆囊收缩素八肽（CCK-8），诱导蛋白激酶D （PKD）激活，在1分钟内检测到，在激素刺激2.5分钟后达到最大值（类似于10倍）。CCK-8和胃泌素的PKD激活均在10 nM处达到半最大值。用不同浓度的选择性PKC抑制剂Ro 31-8220或gf - 1以浓度依赖性的方式有效阻断随后添加CCK-8诱导的PKD激活。我们的研究结果表明，pkc依赖性的PKD激活是胃泌素和CCK-8通过CCK2受体作用的一个新的早期事件。(C) 2001年欧洲生化学会联合会。Elsevier Science B.V.版权所有。
Addition of gastrin or cholecystokinin octapeptide (CCK-8) to cultures of Rat-1 cells stably transfected with the CCK2 (CCKB/gastrin) receptor induced protein kinase D (PKD) activation that was detectable within 1 min and reached a maximum (similar to 10-fold) after 2.5 min of hormonal stimulation. Half-maximal PKD activation for both CCK-8 and gastrin mas achieved at 10 nM. Treatment with various concentrations of the selective PKC inhibitors Ro 31-8220 or GF-I potently blocked PKD activation induced by subsequent addition of CCK-8 in a concentration-dependent fashion. Our results indicate that PKC-dependent PKD activation is a novel early event in the action of gastrin and CCK-8 via CCK2 receptors. (C) 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.