Involvement of the 5'-untranslated region in cold-regulated expression of the rbpA1 gene in the cyanobacterium Anabaena variabilis M3.
Involvement of the 5'-untranslated region in cold-regulated expression of the rbpA1 gene in the cyanobacterium Anabaena variabilis M3.
复制标题
5-非翻译区参与蓝藻多变鱼腥藻 M3 中 rbpA1 基因的冷调节表达。
DOI：
10.1093/nar/26.9.2192
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发表时间：
1998
影响因子：
14.9
通讯作者：
A. Nakamura
中科院分区：
文献类型：
--
作者：
N. Sato;A. Nakamura
在低于28 ℃的低生长温度下，变异鱼腥藻中rbpA 1基因的转录物显著积累。这种积累在16 ℃时最大。利福平完全消除rbpA 1转录的积累，但不是氯霉素。这种冷诱导的积累不需要光合作用。这种转录本的积累部分是由于rbpA 1转录本在低温下的稳定性增加。当几乎整个5 '-非翻译区（5'-UTR）保持未缺失时，含有与lacZ基因融合的3 '-缺失rbpA 1序列的嵌合基因的表达受低温调节。进一步的缺失导致嵌合基因的组成型表达。5 '-UTR序列在体外与来自38 ℃生长的细胞的蛋白质提取物形成两种类型的复合物，但不与来自22 ℃生长的细胞的提取物形成复合物。亲和纯化鉴定了复合物1中75和32 kDa的多肽和复合物2中72 kDa的多肽。这些结果是兼容的模型中，rbpA 1基因的表达是由转录去阻遏在低温下进行调节，虽然其他机制，如mRNA的稳定性调节，也可能有助于温度依赖性调节。
Transcript of the rbpA1 gene in Anabaena variabilis accumulates significantly at low growth temperatures below 28 degreesC. This accumulation was maximal at 16 degreesC. Accumulation of the rbpA1 transcript was completely abolished by rifampicin, but not by chloramphenicol. Photosynthesis was not required for this cold-induced accumulation. This accumulation of transcript was partly accounted for by increased stability of the rbpA1 transcript at low temperature. Expression of chimeric genes containing 3'-deleted rbpA1 sequences fused to the lacZ gene was regulated by low temperature when almost the entire 5'-untranslated region (5'-UTR) remained undeleted. Further deletion resulted in constitutive expression of the chimeric gene. The 5'-UTR sequence formed two types of complexes in vitro with protein extract from cells grown at 38 degreesC, but not with extract from the 22 degreesC grown cells. Affinity purification identified polypeptides of 75 and 32 kDa in Complex 1 and a 72 kDa polypeptide in Complex 2. These results are compatible with a model in which expression of the rbpA1 gene is regulated by transcriptional derepression at low temperature, although additional mechanisms, such as regulation of mRNA stability, might also contribute to temperature-dependent regulation.