Cell-compatible covalently reinforced beads obtained from a chemoenzymatically engineered alginate
Cell-compatible covalently reinforced beads obtained from a chemoenzymatically engineered alginate
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DOI：
10.1016/j.biomaterials.2006.05.011
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发表时间：
2006-09-01
期刊：
影响因子：
14
通讯作者：
Skjak-Braek, Gudmund
中科院分区：
文献类型：
--
作者：
Rokstad, Anne Mari;Donati, Ivan;Skjak-Braek, Gudmund
已经利用化学酶策略来制备具有高稳定性的共价连接的藻酸盐珠。这是通过将甘露糖醛酸（具有100%甘露糖醛酸（M）的藻酸盐）与甲基丙烯酸酯部分接枝，然后以交替序列（MG-嵌段）和G-嵌段进行将M转化为古罗糖醛酸（G）的两个酶促步骤来实现的。以这种方式获得具有更好的凝胶形成能力的甲基丙烯酸酯接枝海藻酸盐。通过使用引发甲基丙烯酸酯部分聚合的光引发系统将共价结合引入到珠中。通过用EDTA处理后保持完整的珠粒证明共价连接。新的化学酶光交联（CEPC）珠与具有低包封后能力的细胞（如C2 C12成肌细胞和人胰岛）相容。包封后胰岛继续分泌胰岛素。相反，具有高包封后增殖能力的细胞如293-endo细胞在包封后2周内死亡。新型CEPC珠粒的卓越稳定性和细胞相容性使其成为未来应用中递送治疗性蛋白质的生物反应器。(c)2006爱思唯尔有限公司保留所有权利。
A chemoenzymatic strategy has been exploited to make covalently linked alginate beads with high stability. This was achieved by grafting mannuronan (alginate with 100% mannuronic acid (M)) with methacrylate moieties and then performing two enzymatic steps converting M to guluronic acid (G) in alternating sequences (MG-blocks) and in G-blocks. In this way a methacrylate grafted alginate with better gel-forming ability was achieved. Covalent bindings were introduced into the beads by using a photoinitiating system that initiated polymerization of the methacrylate moieties. The covalent links were demonstrated by beads remaining intact after treatment with EDTA. The new chemoenzymatic photocrosslinked (CEPC) beads were compatible with cells with low post-encapsulation ability like C2C12 myoblasts and human pancreatic islets. The islets continued secreting insulin after encapsulation. On contrary, cells with a high post-encapsulation proliferative ability like 293-endo cells died within 2-week post-encapsulation. The exceptional stability and the cell compatibility of the new CEPC beads make them interesting as bioreactors for delivering therapeutic proteins in future applications. (c) 2006 Elsevier Ltd. All rights reserved.