A new 2CTAB/PCI method improves DNA amplification success from faeces of Mediterranean (Barbary macaques) and tropical (lowland gorillas) primates
A new 2CTAB/PCI method improves DNA amplification success from faeces of Mediterranean (Barbary macaques) and tropical (lowland gorillas) primates
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DOI：
10.1007/s10592-007-9361-8
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发表时间：
2008-06-01
影响因子：
2.2
通讯作者：
Menard, Nelly
中科院分区：
文献类型：
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作者：
Vallet, Dominique;Petit, Eric J.;Menard, Nelly
当使用适当的提取程序时，可以从粪便中获得质量足以解决种群生物学或行为生态学问题的动物基因组DNA提取物。提取物中PCR抑制剂的存在通常是限制DNA扩增成功的主要因素。我们比较了热带灵长类动物（西部低地大猩猩，大猩猩g.大猩猩）和地中海灵长类动物（巴巴利猕猴，Macaca pacus）之间的广泛用于动物的标准化提取技术（QIAamp（R）粪便试剂盒）、主要用于植物物种的技术（CTAB）和新方案（2CTAB/PCI）。扩增成功率从51%到97%不等，在两个物种中用2CTAB/PCI方案达到最高成功率。
Animal genomic DNA extracts of sufficient quality to address questions about population biology or behavioural ecology can be obtained from faeces when adequate extraction procedures are used. The presence of PCR inhibitors in extract products appears generally the main factor limiting DNA amplification success. We compared DNA amplification success from faeces of a tropical primate (western lowland gorilla, Gorilla g. gorilla) and a Mediterranean primate (Barbary macaque, Macaca sylvanus) between a standardized extraction technique widely used in animals (QIAamp (R) stool kit), a technique mainly used in plant species (CTAB) and a new protocol (2CTAB/PCI). Amplification success varied from 51% to 97%, the highest success being reached with the 2CTAB/PCI protocol in both species.