Inhibition of D(--)-beta-hydroxybutyrate dehydrogenase by butanedione, phenylglyoxal, and diethyl pyrocarbonate.
Inhibition of D(--)-beta-hydroxybutyrate dehydrogenase by butanedione, phenylglyoxal, and diethyl pyrocarbonate.
复制标题
丁二酮、苯基乙二醛和焦碳酸二乙酯对 D(--)-β-羟基丁酸脱氢酶的抑制作用。
DOI：
10.1021/bi00506a002
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
Hatefi,Y
中科院分区：
文献类型：
--
作者：
Phelps,DC;Hatefi,Y
材料和方法制备亚线粒体颗粒，并如本期前一篇论文（Phelps & Hatefi，1981）所述测定蛋白质浓度。除了图例中规定的细节之外，β-羟基丁酸氧化和乙酰乙酸还原的测定也与之前相同（Phelps & Hatefi，1981）。比活性表示为在36-37 ℃下每毫克蛋白质每分钟还原的NAD或氧化的NADH的微摩尔数.苯基乙二醛和丁二酮得自Aldrich Chemical Co.，焦碳酸二乙酯得自Sigma Chemical Co.。其它化学品的来源与以前相同（Phelps & Hatefi，1981）。
Materials and MethodsSubmitochondrial particles were prepared and protein concentrations determined as in the preceding paper of this issue (Phelps & Hatefi, 1981). Except for the details specified in the figure legends, the assays for/3-hydroxybutyrate oxidation and acetoacetate reduction were also the same as before (Phelps & Hatefi, 1981). Specific activity is expressed as micromoles of NAD reduced or NADH oxidized per minute per milligram of protein at 36-37 C. Phenylglyoxal and butanedione were obtained from Aldrich Chemical Co., and diethyl pyrocarbonate was obtained from Sigma Chemical Co. The sources of other chemicals were the same as before (Phelps & Hatefi, 1981).