Neuromuscular electrical stimulation of the hindlimb muscles for movement therapy in a rodent model.
Neuromuscular electrical stimulation of the hindlimb muscles for movement therapy in a rodent model.
复制标题
DOI：
10.1016/j.jneumeth.2008.09.015
复制
发表时间：
2009-01-30
影响因子：
3
通讯作者：
Jung, Ranu
中科院分区：
文献类型：
--
作者：
Ichihara, Kazuhiko;Venkatasubramanian, Ganapriya;Abbas, James J.;Jung, Ranu
关键词：
神经肌肉电刺激（NMES）可以为中枢神经系统损伤后的人提供功能性运动。长期NMES诱导的重复肢体运动的神经可塑性影响还没有得到很好的理解。可以实施NMES的神经创伤啮齿动物模型可能对此类调查有效。我们提出了一个啮齿动物模型的屈肌和伸肌的髋关节，膝关节和踝关节后肢肌肉的NMES。在10只成年雌性Long Evans大鼠的目标肌肉的识别运动点附近植入用于啮齿动物的定制制造的肌内刺激电极。使用强度持续时间曲线、等长关节扭矩募集曲线和关节角度测量表征改变NMES脉冲刺激参数的影响。数据表明，当电流用作控制参数时，短脉冲宽度具有产生分级扭矩招募曲线的优点。75Hz或更高的刺激频率产生融合收缩。数据证明了准确植入电极并获得选择性、分级、可重复、强烈肌肉收缩的能力。通过刺激目标肌肉，可以获得与在相同啮齿动物物种中正常跑步机行走中获得的膝关节和踝关节角度偏移相当的膝关节和踝关节角度偏移。获得的关节扭矩（归一化体重）大于文献报道的小尾兽目哺乳动物和峰值等距踝跖屈在不同的啮齿类动物。该模型系统可用于NMES辅助后肢运动治疗的研究。
Neuromuscular electrical stimulation (NMES) can provide functional movements in people after central nervous system injury. The neuroplastic effects of long-term NMES induced repetitive limb movement are not well understood. A rodent model of neurotrauma in which NMES can be implemented may be effective for such investigations. We present a rodent model for NMES of the flexor and extensor muscles of the hip, knee, and ankle hindlimb muscles. Custom fabricated intramuscular stimulating electrodes for rodents were implanted near identified motor points of targeted muscles in ten adult, female Long Evans rats. The effects of altering NMES pulse stimulation parameters were characterized using strength duration curves, isometric joint torque recruitment curves and joint angle measures. The data indicate that short pulse widths have the advantage of producing graded torque recruitment curves when current is used as the control parameter. A stimulus frequency of 75Hz or more produces fused contractions. The data demonstrate ability to accurately implant the electrodes and obtain selective, graded, repeatable, strong muscle contractions. Knee and ankle angular excursions comparable to those obtained in normal treadmill walking in the same rodent species can be obtained by stimulating the target muscles. Joint torques (normalized to body weight) obtained were larger than those reported in the literature for small tailed therian mammals and for peak isometric ankle plantarflexion in a different rodent species. This model system could be used for investigations of NMES assisted hindlimb movement therapy.
登录
查看更多内容
影响因子：
2.4
作者：
Bajd, T;Kralj, A;Lavrac, N
通讯作者：
Lavrac, N
影响因子：
2.5
作者：
Gorassini, M;Eken, T;Hultborn, H
通讯作者：
Hultborn, H
影响因子：
8.3
作者：
Daly, JJ;Roenigk, K;Ruff, RL
通讯作者：
Ruff, RL
影响因子：
4.3
作者：
Field-Fote, EC
通讯作者：
Field-Fote, EC
影响因子：
3.4
作者：
Beaumont, E;Houlé, JD;Gardiner, PF
通讯作者：
Gardiner, PF