Reciprocal interference between specific CJD and scrapie agents in neural cell cultures
Reciprocal interference between specific CJD and scrapie agents in neural cell cultures
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DOI：
10.1126/science.1118155
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发表时间：
2005-10-21
期刊：
影响因子：
56.9
通讯作者：
Manuelidis, L
中科院分区：
文献类型：
--
作者：
Nishida, N;Katamine, S;Manuelidis, L
用减毒克雅氏病因子（SY-CJD）感染小鼠会干扰毒性更强的人源性克雅氏病因子（FU-CJD）的重复感染，并且不需要病理性朊蛋白（PrPres）。使用一个快速的共培养系统，我们发现，一个神经细胞系的免疫系统细胞的自由同样支持大量的CJD剂干扰没有PrPres。此外，SY-CJD预防羊源性钱德勒（Ch）和22 L羊瘙痒症因子的重复感染。然而，只有22 L，而不是Ch防止FU-CJD感染，即使两个瘙痒症菌株引起丰富的PrPres。羊源性和人源性病原体的特定菌株之间的这种关系可能会影响其流行和流行病传播。
Infection of mice with an attenuated Creutzfetdt-Jakob disease agent (SY-CJD) interferes with superinfection by a more virulent human-derived CJD agent (FU-CJD) and does not require pathological prion protein (PrPres). Using a rapid coculture system, we found that a neural cell line free of immune system cells similarly supported substantial CJD agent interference without PrPres. In addition, SY-CJD prevented superinfection by sheep-derived Chandler (Ch) and 22L scrapie agents. However, only 22L and not Ch prevented FU-CJD infection, even though both scrapie strains provoked abundant PrPres. This relationship between particular strains of sheep- and human-derived agents is likely to affect their prevalence and epidemic spread.