Cell type-specific structural plasticity of axonal branches and boutons in the adult neocortex
Cell type-specific structural plasticity of axonal branches and boutons in the adult neocortex
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DOI：
10.1016/j.neuron.2006.02.017
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发表时间：
2006-03-16
期刊：
影响因子：
16.2
通讯作者：
Svoboda, K
中科院分区：
文献类型：
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作者：
De Paola, V;Holtmaat, A;Svoboda, K
我们对小鼠桶状皮层 (L) 1 层的轴突进行体内成像。丘脑和 L2/3/5 或 L6 锥体细胞的轴突根据其不同的形态进行鉴定。它们的分支模式和大小在几个月内保持稳定。然而，轴突分支和纽扣显示出细胞类型特异性的重排。丘脑皮质传入神经的结构可塑性主要是由于分支的伸长和回缩（范围为 4 天内 1-150 μm；类似于总轴突长度的 5%），而大多数 bouton 持续长达 9 个月（持续超过 1 个月类似于 85%）。相比之下，L6 轴突末端纽扣具有高度可塑性（1 个月以上的持续性类似于 40%），而其他皮质内轴突纽扣则显示出中等水平的可塑性。回顾性电子显微镜显示，新的纽扣会产生突触。我们的数据表明，轴突分支和纽扣的结构可塑性有助于特定功能电路的重塑。
We imaged axons in layer (L) 1 of the mouse barrel cortex in vivo. Axons from thalamus and L2/3/5, or L6 pyramidal cells were identified based on their distinct morphologies. Their branching patterns and sizes were stable over times of months. However, axonal branches and boutons displayed cell type-specific rearrangements. Structural plasticity in thalamocortical afferents was mostly due to elongation and retraction of branches (range, 1-150 mu m over 4 days; similar to 5% of total axonal length), while the majority of boutons persisted for up to 9 months (persistence over 1 month similar to 85%). In contrast, L6 axon terminaux boutons were highly plastic (persistence over 1 month similar to 40 %), and other intracortical axon boutons showed intermediate levels of plasticity. Retrospective electron microscopy revealed that new boutons make synapses. Our data suggest that structural plasticity of axonal branches and boutons contributes to the remodeling of specific functional circuits.