Solid-Phase Synthesis of Sequence-Defined T-, i-, and U-Shape Polymers for pDNA and siRNA Delivery
Solid-Phase Synthesis of Sequence-Defined T-, i-, and U-Shape Polymers for pDNA and siRNA Delivery
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DOI：
10.1002/anie.201102165
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发表时间：
2011-01-01
影响因子：
16.6
通讯作者：
Wagner, Ernst
中科院分区：
文献类型：
--
作者：
Schaffert, David;Troiber, Christina;Wagner, Ernst
图S2. A）在16的N/P比和不同FBS血清浓度下，通过琼脂糖凝胶位移测定法测定血清中的DNA多聚物稳定性。DNA多聚物形成（在20 mM HEPES pH 7.4中）和孵育时间（30分钟）如体内实验那样进行。在颗粒形成后将血清加入到聚合复合物中，并将样品在室温下孵育15分钟。具有80的聚合复合物是完全稳定的，具有78的聚合复合物观察到一些非常中度的不稳定（涂片）。裸DNA（“DNA”）在最高FBS浓度下显示出一些降解（超螺旋形式的消失）和一些阻滞
Figure S2. A) DNA polyplex stability in serum determined by agarose gel shift assay at N/P ratio of 16 and different FBS serum concentrations. DNA polyplex formation (in 20 mM HEPES pH 7.4) and incubation time (30 min) was performed as for the in vivo experiments. Serum was added to the polyplexes after particle formation and the samples were incubated for 15 min at room temperature. Polyplexes with 80 were completely stable, with 78 some very moderate destabilization (smear) was observed. Naked DNA (“DNA”) shows some degradation (disappearance of supercoiled form) and some retardation at highest FBS concentration