Molecular epidemiology of bluetongue virus in Portugal during 2004-2006 outbreak
Molecular epidemiology of bluetongue virus in Portugal during 2004-2006 outbreak
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DOI：
10.1016/j.vetmic.2007.04.014
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发表时间：
2007-09-20
影响因子：
3.3
通讯作者：
Fevereiro, Miguel
中科院分区：
文献类型：
--
作者：
Barros, Silvia C.;Ramos, Fernanda;Fevereiro, Miguel
经过44年的流行病学沉默，蓝舌病病毒（BTV）于2004年秋季重新引入葡萄牙。第一例蓝舌病（BT）临床病例是在葡萄牙南部靠近西班牙边境的羊场报告的。在2004-2006年的流行病期间，从绵羊和牛中共分离到6株BTV，5株血清型4和1株血清型2。测定了BTV-4型原型株（BTV 4/22045/PT 04）和BTV-2型原型株（BTV 2/26629/PT 05）L2、S7和S10基因片段的核苷酸序列，并与世界其他地区的BTV-4型原型株（BTV 4/22045/PT 04）和BTV-2型原型株（BTV 2/26629/PT 05）进行了比较。系统发育分析表明，BTV 4/22045/PT 04与1998年以来在地中海盆地流行的其他BTV-4株相关，与2003年从撒丁岛和科西嘉分离的BTV-4株显示出最高的同源性（99%），而BTV 2/26629/PT 05与Onderstepoort BTV-2直播几乎无法区分，减毒疫苗株及其在意大利分离的相关野毒株。由于葡萄牙从未使用过BTV-2减毒活疫苗，因此该毒株的分离可能代表了地中海欧洲其他国家使用的疫苗病毒的自然循环。(c)2007 Elsevier B. V.保留所有权利。
After 44 years of epidemiological silence, bluetongue virus (BTV) was reintroduced in Portugal in the autumn of 2004. The first clinical cases of bluetongue disease (BT) were notified in sheep farms located in the South of Portugal, close to the Spanish border. A total of six BTV, five of serotype 4 and one of serotype 2 were isolated from sheep and cattle during the 2004-2006 epizootics. The nucleotide sequence of gene segments L2, S7 and S 10 of BTV-4 prototype strain (BTV4/22045/PT04) obtained from the initial outbreak and of BTV-2 (BTV2/26629/PT05) was fully determined and compared with those from other parts of the world. The phylogenetic analysis revealed that BTV4/22045/PT04 is related to other BTV-4 strains that circulate in the Mediterranean basin since 1998, showing the highest identity (99%) with BTV-4 isolates of 2003 from Sardinia and Corsica, whereas BTV2/26629/PT05 is almost indistinguishable from the Onderstepoort BTV-2 live-attenuated vaccine strain and its related field strain isolated in Italy. Since live-attenuated BTV-2 vaccine was never used in Portugal, the isolation of this strain may represent a natural circulation of the vaccine virus used in other countries in Mediterranean Europe. (c) 2007 Elsevier B.V. All rights reserved.