SOUTHERN ANALYSIS OF HUMAN HEAD AND NECK-CANCER CELLS FOR HOMOLOGOUS SEQUENCES USING YEAST-GAMMA REPAIR GENES
SOUTHERN ANALYSIS OF HUMAN HEAD AND NECK-CANCER CELLS FOR HOMOLOGOUS SEQUENCES USING YEAST-GAMMA REPAIR GENES
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DOI：
10.1002/jso.2930380410
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发表时间：
1988-08-01
影响因子：
2.5
通讯作者：
WEICHSELBAUM, RR
中科院分区：
文献类型：
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作者：
BECKETT, MA;WEICHSELBAUM, RR
我们使用Southern分析来检查酵母基因RAD 51、RAD 52、RAD 54和RAD 55之间的同源性，以寻找抗辐射或修复熟练的人类肿瘤细胞系和正常胎盘DNA中可能的伽马修复基因。没有观察到同源性；然而，包括进一步的基因限制和转基因在内的其他策略正在进行中，以确定人类肿瘤中的修复基因。了解放射修复的机制可能会导致更有效的临床放射治疗方案。
We employed southern analysis to examine homology between yeast genes RAD 51, RAD 52, RAD 54, and RAD 55 for possible gamma repair genes in radioresistant or repair proficient human tumor cell lines and normal placental DNA. No homology was observed; however, other strategies including further gene restriction and transfection are underway to identify repair genes in human tumors. Understanding mechanisms of radiation repair might lead to more effective clinical radiation treatment protocols.