Tat peptide as an efficient molecule to translocate gold nanoparticles into the cell nucleus
Tat peptide as an efficient molecule to translocate gold nanoparticles into the cell nucleus
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DOI：
10.1021/bc050033
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发表时间：
2005-09-01
影响因子：
4.7
通讯作者：
Berry, CC
中科院分区：
文献类型：
--
作者：
de la Fuente, JM;Berry, CC
用纳米颗粒标记靶向分子已经彻底改变了通过电子显微镜观察细胞或组织成分的方法。一个特别理想的目标是细胞核，因为遗传信息就在那里。迄今为止，利用纳米颗粒进行核靶向尚未被证明是非常成功的，由于质膜和核膜的不可渗透性;因此纳米颗粒的设计和合成是一个关键因素。在这篇文章中，我们报告了一个简单的和经济的方法合成功能化的达特蛋白衍生肽序列的水溶性金纳米粒子。随后通过光学和透射电子显微镜在体外用人成纤维细胞系测试颗粒，以确定这些纳米颗粒的生物相容性以及用易位肽的功能化是否允许颗粒转移穿过细胞膜并定位在细胞核中。
The labeling of targeting molecules with nanoparticles has revolutionized the visualization of cellular or tissue components by electron microscopy. A particularly desirable target is the nucleus, because the genetic information is there. To date, utilizing nanoparticles for nuclear targeting has not proved very successful due to the impermeable nature of the plasma and nuclear membranes; thus nanoparticle design and synthesis is a critical factor. We report in this article the synthesis of water-soluble gold nanoparticles functionalized with a Tat protein-derived peptide sequence by a straightforward and economical methodology. The particles were subsequently tested in vitro with a human fibroblast cell line by optical and transmission electron microscopy to determine the biocompatibility of these nanoparticles and whether the functionalization with the translocation peptide allowed particles to transfer across the cell membrane and locate in the nucleus.