Role of kidney tissue in the production of 25-hydroxyvitamin D3-26,23-lactone and 1 alpha, 25-dihydroxyvitamin D3-26,23-lactone.
Role of kidney tissue in the production of 25-hydroxyvitamin D3-26,23-lactone and 1 alpha, 25-dihydroxyvitamin D3-26,23-lactone.
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肾组织在 25-羟基维生素 D3-26,23-内酯和 1 α,25-二羟基维生素 D3-26,23-内酯生产中的作用。
DOI：
10.1073/pnas.77.11.6411
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发表时间：
1980
影响因子：
11.1
通讯作者：
DeLuca,HF
中科院分区：
文献类型：
--
作者：
Tanaka,Y;Wichmann,JK;Paaren,HE;Schnoes,HK;DeLuca,HF
标记的25-羟基维生素D3- 26，23-内酯是从处死前给予[3 α-3H]-25-羟基维生素D324小时的维生素D充足大鼠的血清中分离的。通过与真实内酯在直相和反相高效液相色谱上的共色谱法鉴别代谢产物。通过肾切除术消除了内酯的产生，表明肾脏是合成的部位。从给予大剂量维生素D的鸡的肾脏匀浆可以进行从25-羟基维生素D3的内酯的体外生产。当使用1 α，25-二羟基维生素D3作为底物时，该系统仅产生被认为是1 α，25-二羟基维生素D3- 26，23-内酯的痕量化合物。然而，将佝偻病鸡肾匀浆与25-羟基维生素D3- 26，23-内酯一起孵育产生了大量的化合物，该化合物已通过质谱法鉴定为1 α，25-二羟基维生素D3- 26，23-内酯。因此，C-26上的官能团的发展和最终的内酯形成通过作用于25-羟基维生素D3的系统在肾脏中发生。
Labeled 25-hydroxyvitamin D3-26,23-lactone was isolated from the serum of vitamin D-repleted rats given [3 alpha-3H]-25-hydroxyvitamin D324 hr prior to sacrifice. The metabolite was identified by cochromatography with the authentic lactone on straight-phase and reversed-phase high-performance liquid chromatography. Production of the lactone was abolished by nephrectomy indicating that the kidney is the site of synthesis. Homogenate of kidneys from chickens given large doses of vitamin D can carry out in vitro production of the lactone from 25-hydroxyvitamin D3. When 1 alpha, 25-dihydroxyvitamin D3 was used as substrate, this system produced only traces of a compound believed to be 1 alpha, 25-dihydroxyvitamin D3-26,23-lactone. However, incubation of rachitic chicken kidney homogenates with 25-hydroxyvitamin D3-26,23-lactone produced substantial amounts of a compound that has been identified by mass spectrometry as 1 alpha, 25-dihydroxyvitamin D3-26,23-lactone. Thus, the development of a functional group on C-26 and eventual lactone formation takes place in kidney by a system acting on 25-hydroxyvitamin D3.