Isolation and Culture of Microglia.
Isolation and Culture of Microglia.
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小胶质细胞的隔离和培养。
DOI：
10.1002/cpim.70
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发表时间：
2019-06
影响因子：
--
通讯作者：
Bennett ML
中科院分区：
文献类型：
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作者：
Bohlen CJ;Bennett FC;Bennett ML
小胶质细胞占中枢神经系统（CNS）细胞的5 - 10%，对神经组织的发育、内稳态、损伤和修复都有作用。作为中枢神经系统的组织驻留巨噬细胞，小胶质细胞执行核心的先天免疫功能，例如病原体/损伤的检测、细胞因子分泌和吞噬作用。然而，小胶质细胞及其神经环境所特有的其他特性正开始被人们认识。本单元介绍了使用小胶质细胞特异性表面标记通过荧光激活细胞分选（FACS）纯化小胶质细胞的方法，或者通过磁分选和免疫淘选富集小胶质细胞的方法。我们还提供了从不同发育阶段培养原代小胶质细胞的详细信息。自始至终，我们关注处理小胶质细胞的特殊注意事项，并比较不同方案的相对优势或劣势。
Microglia represent 5–10% of cells in the central nervous system (CNS) and contribute to the development, homeostasis, injury, and repair of neural tissues. As the tissue-resident macrophages of the CNS, microglia execute core innate immune functions such as detection of pathogens/damage, cytokine secretion, and phagocytosis. However, additional properties that are specific to microglia and their neural environment are beginning to be appreciated. This unit describes approaches for purification of microglia by Fluorescence-Activated Cell Sorting (FACS) using microglia-specific surface markers, or for enrichment of microglia by magnetic sorting and immunopanning. We also provide detailed information about culturing primary microglia from various developmental stages. Throughout, we focus on special considerations for handling microglia and compare the relative strengths or disadvantages of different protocols.