Germ-line induction of the Caenorhabditis elegans vulva.
Germ-line induction of the Caenorhabditis elegans vulva.
复制标题
秀丽隐杆线虫外阴的种系诱导。
DOI：
10.1073/pnas.0510264103
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发表时间：
2006
影响因子：
11.1
通讯作者：
Kimble,Judith
中科院分区：
文献类型：
--
作者：
Thompson,BethE;Lamont,LianaB;Kimble,Judith
Development of theCaenorhabditis elegansvulva serves as a paradigm for intercellular signaling during animal development. In wild-type animals, the somatic gonadal anchor cell generates the LIN-3/EGF ligand to induce vulval fates in the underlying hypodermis, whereas FBF, FOG-1, and FOG-3 control germ-line development. Here we report that FBF functions redundantly with FOG-1 and FOG-3 to control vulval induction: animals lacking FBF and either FOG-1 or FOG-3 have multiple vulvae, the Muv phenotype. Thefog; fbfMuv phenotype is generated by aberrant induction of vulval precursor cells (VPCs): in wild-type animals, three VPCs are induced to form a single vulva, but, infog; fbfmutants, four or five VPCs are typically induced, resulting in ectopic vulvae. Laser ablation experiments and mosaic analyses demonstrate that the germ line is critical for thefog; fbfMuv phenotype. Consistent with that site of action, we detect FBF and FOG-1 in the germ line but not in the VPCs. The simplest interpretation is that FOG-1, FOG-3, and FBF act in the germ line to influence vulval fates. The LIN-3/EGF ligand may be the germ-line signal to the VPCs: thefog; fbfMuv phenotype depends on LIN-3 activity, and thelin-33′ UTR possesses an FBF binding element. Our findings reveal new insights into germ line-to-soma signals and the role of PUF proteins in animal development.
