Gene knockout study reveals that cytosolic ascorbate peroxidase 2(OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses.
Gene knockout study reveals that cytosolic ascorbate peroxidase 2(OsAPX2) plays a critical role in growth and reproduction in rice under drought, salt and cold stresses.
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基因敲除研究揭示细胞质抗坏血酸过氧化物酶 2 (OsAPX2) 在干旱、盐和冷胁迫下的水稻生长和繁殖中发挥关键作用
DOI：
10.1371/journal.pone.0057472
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Lu T
中科院分区：
文献类型：
--
作者：
Zhang Z;Zhang Q;Wu J;Zheng X;Zheng S;Sun X;Qiu Q;Lu T
植物抗坏血酸过氧化物酶（APXs）是催化H2 O2歧化为H2O和O2的酶，在植物体内活性氧平衡中起着重要作用。水稻基因组中有8个OsAPX，但它们的生理功能仍有待确定。本研究利用T-DNA敲除突变体研究了OsAPX 2基因在干旱、盐胁迫和低温胁迫下的功能。利用20%PEG-2000对水稻T-DNA插入文库进行遗传筛选，获得Osapx 2基因敲除突变体。OsAPX 2功能缺失影响水稻幼苗的生长发育，导致幼苗半矮化、叶片黄绿、叶片拟病斑和种子不育。OsAPX 2的表达是发育和空间调控的，并诱导干旱，盐，冷胁迫。Osapx 2突变体APX活性较低，对非生物胁迫敏感; OsAPX 2过表达可提高APX活性，增强抗逆性。胁迫处理后，相对于野生型植物，Osapx 2突变体中的H2 O2和MDA水平高，但OsAPX 2-OX转基因株系中的H2 O2和MDA水平低。综上所述，OsAPX 2通过清除活性氧保护水稻幼苗免受非生物胁迫，在水稻生长发育中发挥重要作用。
Plant ascorbate peroxidases (APXs), enzymes catalyzing the dismutation of H2O2 into H2O and O2, play an important role in reactive oxygen species homeostasis in plants. The rice genome has eight OsAPXs, but their physiological functions remain to be determined. In this report, we studied the function of OsAPX2 gene using a T-DNA knockout mutant under the treatment of drought, salt and cold stresses. The Osapx2 knockout mutant was isolated by a genetic screening of a rice T-DNA insertion library under 20% PEG-2000 treatment. Loss of function in OsAPX2 affected the growth and development of rice seedlings, resulting in semi-dwarf seedlings, yellow-green leaves, leaf lesion mimic and seed sterility. OsAPX2 expression was developmental- and spatial-regulated, and was induced by drought, salt, and cold stresses. Osapx2 mutants had lower APX activity and were sensitive to abiotic stresses; overexpression of OsAPX2 increased APX activity and enhanced stress tolerance. H2O2 and MDA levels were high in Osapx2 mutants but low in OsAPX2-OX transgenic lines relative to wild-type plants after stress treatments. Taken together, the cytosolic ascorbate peroxidase OsAPX2 plays an important role in rice growth and development by protecting the seedlings from abiotic stresses through scavenging reactive oxygen species.
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