The BANYULS gene encodes a DFR-like protein and is a marker of early seed coat development
The BANYULS gene encodes a DFR-like protein and is a marker of early seed coat development
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DOI：
10.1046/j.1365-313x.1999.00529.x
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发表时间：
1999-08-01
期刊：
影响因子：
7.2
通讯作者：
Delseny, M
中科院分区：
文献类型：
--
作者：
Devic, M;Guilleminot, J;Delseny, M
BANYULS（BAN）基因突变导致拟南芥未成熟种皮中花青素的早熟积累。ban-1等位基因已从一组T-DNA转化体中分离出来，并被整合分子标记。野生型和两个独立的突变等位基因的测序证实了该基因的身份。全长cDNA序列的分析揭示了一个开放的阅读框架，编码一个342个氨基酸的蛋白质，该蛋白质与DFR和苯丙烷类生物合成途径的其他酶具有很强的相似性。BAN的表达被限制在未成熟种子的内皮细胞在前球形到早期球形的发展阶段，从母系遗传的表型预测，因此代表了早期分化和发展的种皮的标记。BAN可能参与种皮中花青素和原花青素的产生之间的代谢通道。
Mutations in the BANYULS (BAN) gene lead to precocious accumulation of anthocyanins in immature seed coat in Arabidopsis. The ban -1 allele has been isolated from a collection of T-DNA transformants and found to be tagged by the integrative molecule. The sequencing of wild-type and two independent mutant alleles confirmed the identity of the gene. Analysis of the full-length cDNA sequence revealed an open reading frame encoding a 342 amino acid protein which shared strong similarities with DFR and other enzymes of the phenylpropanoid biosynthesis pathway. BAN expression was restricted to the endothelium of immature seeds at the pre-globular to early globular stages of development as predicted from the maternal inheritance of the phenotype, and therefore represents a marker for early differentiation and development of the seed coat. BAN is probably involved in a metabolic channelling between the production of anthocyanins and pro-anthocyanidins in the seed coat.