Apical loop-internal loop interactions:: A new RNA-RNA recognition motif identified through in vitro selection against RNA hairpins of the hepatitis C virus mRNA
Apical loop-internal loop interactions:: A new RNA-RNA recognition motif identified through in vitro selection against RNA hairpins of the hepatitis C virus mRNA
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DOI：
10.1021/bi0121508
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发表时间：
2002-05-07
期刊：
影响因子：
2.9
通讯作者：
Toulmé, JJ
中科院分区：
文献类型：
--
作者：
Aldaz-Carroll, L;Tallet, A;Toulmé, JJ
d我们进行了寡核糖核苷酸的体外选择，以鉴定高亲和力的基序，识别位于丙型肝炎病毒mRNA的3'端（SL 1）和5'端（内部核糖体进入位点的结构域IV）的RNA发夹。我们选择了由与SL 1顶端环互补的内环构成的适体，其两侧是富含G-C的双链区域，能够在接近细胞内的离子条件下在37 ℃下形成K（d）为70 nM的复合物。该复合物涉及选择性顶端环（SL 1）-内部环（适体）相互作用。类似的结构组织的适体被独立地鉴定为针对结构域IV，并且显示也产生这样的复合物。顶环-内环相互作用可能构成一个新的识别基序，允许特异性的分子内或分子间RNA-RNA缔合。
dWe performed in vitro selection of oligoribonucleotides in order to identify high-affinity motifs recognizing RNA hairpins located at the 3' end (SL1) and at the 5' end (domain IV of the internal ribosome entry site) of the hepatitis C virus mRNA. We selected aptamers constituted by an internal loop complementary to the SL1 apical loop, flanked by G-C-rich double-stranded regions, able to form complexes with a K(d) of 70 nM, at 37 degreesC under ionic conditions close to intracellular ones. The complex involves selective apical loop (SL1)-internal loop (aptamer) interactions. Similar structurally organized aptamers were independently identified against domain IV and were shown to also give rise to such complexes. Apical loop-internal loop interaction could constitute a new recognition motif allowing specific intra- or intermolecular RNA-RNA association.