Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype.
Isolation of adipose-derived stem cells and their induction to a chondrogenic phenotype.
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脂肪衍生的干细胞的分离及其诱导对软骨表型的诱导。
DOI：
10.1038/nprot.2010.81
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发表时间：
2010-07
期刊：
影响因子：
14.8
通讯作者：
中科院分区：
文献类型：
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作者：
分离、扩增和分化成体干细胞成软骨系的能力是组织工程方法发展的重要一步，用于软骨修复或再生，用于关节损伤或骨关节炎的治疗，或用于整形或重建手术。脂肪源性干细胞（ASCs）为这种再生方法提供了丰富且容易获得的成体干细胞来源。本方案描述了从吸脂液中分离ASCs，以及基于生长因子诱导ASCs成为软骨细胞样细胞的细胞培养条件。这些方法与用于骨髓间充质干细胞的方法相似，但由于ASCs的独特性质而有所不同。研究者可以期待一致的ASC分化，允许由于供体和血清的影响而产生轻微的变化。ASC的分离和软骨细胞样细胞的表征大约需要10-12周，这也被描述。
The ability to isolate, expand, and differentiate adult stem cells into a chondrogenic lineage is an important step in the development of tissue engineering approaches for cartilage repair or regeneration for the treatment of joint injury or osteoarthritis, or for application in plastic or reconstructive surgery. Adipose-derived stem cells (ASCs) provide an abundant and easily accessible source of adult stem cells for use in such regenerative approaches. This protocol describes the isolation of ASCs from liposuction aspirate, as well as cell culture conditions for growth factor based induction of ASCs into chondrocyte-like cells. These methods are similar to those used for bone marrow mesenchymal stem cells but distinct due to the unique properties of ASCs. Investigators can expect consistent ASC differentiation, allowing for slight variation due to donor and serum lot effects. Approximately 10–12 weeks are needed for ASC isolation and the characterization of chondrocyte-like cells, which is also described.