Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system
Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system
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DOI：
10.1016/j.bios.2021.113961
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发表时间：
2022-01-10
影响因子：
12.6
通讯作者：
Denizli, Adil
中科院分区：
文献类型：
--
作者：
Bakhshpour, Monireh;Chiodi, Elisa;Denizli, Adil
考虑到众所周知的传统检测技术的局限性，创新研究的重点是开发新型传感器，为生物分子相互作用提供无标记、高灵敏度、实时、低成本和快速检测。在本研究中，我们通过使用干涉反射成像传感器 (IRIS) 系统的实时无标记动力学测量来演示水溶液中的免疫球蛋白 G (IgG) 检测。通过动力学表征实验，对传感器的性能进行了综合评价，在1-50 μg/mL的IgG浓度范围内获得了较高的相关系数值（>0.94）和较低的检测限（0.25 μg/mL或1.67 nM）。此外，高灵敏度成像系统可确保记录的结合事件的准确量化和可靠验证，为临床应用的直接生物标志物检测提供新的视角。
Considering the limitations of well-known traditional detection techniques, innovative research studies have focused on the development of new sensors to offer label-free, highly sensitive, real-time, low-cost, and rapid detection for biomolecular interactions. In this study, we demonstrate immunoglobulin G (IgG) detection in aqueous solutions by using real-time and label-free kinetic measurements of the Interferometric Reflectance Imaging Sensor (IRIS) system. By performing kinetic characterization experiments, the sensor's performance is comprehensively evaluated and a high correlation coefficient value (>0.94) is obtained in the IgG concentration range of 1-50 mu g/mL with a low detection limit (0.25 mu g/mL or 1.67 nM). Moreover, the highly sensitive imaging system ensures accurate quantification and reliable validation of recorded binding events, offering new perspectives in terms of direct biomarker detection for clinical applications.